plants
Review
Scribbling the Cat: A Case of the “Miracle” Plant,
Moringa oleifera
Thulani Tshabalala 1,2 , Bhekumthetho Ncube 1 , Ntakadzeni Edwin Madala 3 ,
Trevor Tapiwa Nyakudya 4,5 , Hloniphani Peter Moyo 6 , Mbulisi Sibanda 2 and
Ashwell Rungano Ndhlala 1,7, *
1
2
3
4
5
6
7
*
Agricultural Research Council (ARC), Vegetable and Ornamental Plants (VOP), Private Bag X923,
Pretoria 0001, South Africa; tshabalalat1@arc.agric.za (T.T.); ncubeb@arc.agric.za (B.N.)
School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal Pietermaritzburg,
Private Bag X01, Scottsville 3209, South Africa; sibandam3@ukzn.ac.za
Department of Biochemistry, School of Mathematical and Natural Sciences, University of Venda,
Private Bag X5050, Thohoyandou, 0950, South Africa; ntaka.madala@univen.ac.za
Department of Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria,
South Africa; trevortn@gmail.com
Department of Human Anatomy and Physiology, Faculty of Health Sciences, University of Johannesburg,
Doornfontein, Johannesburg 2002, South Africa
Agency for Technical Cooperation and Development (ACTED), Amman, Jordan; hmthunzi@gmail.com
Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences,
University of South Africa, Private Bag X6, Florida 1710, South Africa
Correspondence: NdhlalaA@arc.agric.za; Tel.: +27-12-808-8000
Received: 8 October 2019; Accepted: 31 October 2019; Published: 15 November 2019
Abstract: This paper reviews the properties of the most cultivated species of the Moringaceae family,
Moringa oleifera Lam. The paper takes a critical look at the positive and the associated negative
properties of the plant, with particular emphasis on its chemistry, selected medicinal and nutritional
properties, as well as some ecological implications of the plant. The review highlights the importance
of glucosinolates (GS) compounds which are relatively unique to the Moringa species family, with
glucomoriginin and its acylated derivative being the most abundant. We highlight some new research
findings revealing that not all M. oleifera cultivars contain an important flavonoid, rutin. The review
also focuses on phenolic acids, tannin, minerals and vitamins, which are in high amounts when
compared to most vegetables and fruits. Although there are numerous benefits of using M. oleifera for
medicinal purposes, there are reports of contraindications. Nonetheless, we note that there are no
major harmful effects of M. oleifera that have been reported by the scientific community. M. oleifera is
suspected to be potentially invasive and moderately invasive in some regions of the world because
of its ability to grow in a wide range of environmental conditions. However, the plant is currently
classified as a low potential invasive species and thus there is a need to constantly monitor the species.
Despite the numerous benefits associated with the plant, there is still a paucity of data on clinical
trials proving both the positive and negative effects of the plant. We recommend further clinical trials
to ascertain the properties associated with the plant, especially regarding long term use.
Keywords: allelopathy; glucomoriginin; glucosinolates; invasive species; Moringaceae
1. Introduction
Up to about 80% of the world’s population use natural remedies such as herbs for medication,
mainly because of the ease of accessibility, affordability and most of all, because of safe therapeutics [1].
Traditionally, plants produce secondary metabolites as an adaptive defence mechanism against a broad
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spectrum of potentially damaging biotic and abiotic factors such as pathogens and the environment.
Humans have thus exploited the properties of this biogenic resource (secondary metabolites) in their
fight against human pathogenic microbes [2]. Through their diverse chemical structures, man has
explored and exploited plant secondary metabolites beyond their obvious antimicrobial properties,
especially for human conditions such as cancer, diabetes, inflammation, cardiovascular, etc. The past
decades have seen several plants exploited for their phytoconstituents in either the development of
medicine or nutritional purposes. One such plant has been Moringa oleifera Lam., which is known
to possess a wide spectrum of metabolites with purported nutritional and medicinal properties [3].
This plant is commonly known as the “miracle tree” due to its purported healing powers across the
different spectrum of diseases.
Moringa oleifera belongs to the Moringaceae family (order Brassicales). The Moringaceae family
has a total of 13 species and M. oleifera is the most utilised and cultivated species [4,5]. The plant is
naturally occurring in the north-eastern parts of India. However, due to its ability to grow in a wide
range of conditions, it is now widely cultivated in the tropic and subtropical regions of the world [4].
The plant can grow up to 2 m in the first year and up to 12 m when mature and bears long, drumstick
shaped pods within the first year [6,7]. The common names given to this tree include horseradish
tree because of the taste of the roots and drumstick tree because of the shape of the fruits pods on the
tree. The plant is referred to as the “miracle tree” because of the enormous positive impact it has on
people’s livelihoods. It is reported that every plant part (seeds, flowers, stems, leaves and roots) is
a great source of nutrients and produces major essential medicinal principles [8–11], curing a range
of diseases [12,13]. For industrial uses, biodiesel and cosmetic oil can be extracted from the seeds.
The seeds can also be used in water purification processes [14,15].
Moringa oleifera is one of the plants with a great phytochemical profile, and it is considered to
be in the top 10% out of 500,000 species being used for conventional medicines [16]. With so much
attention given to the plant, there is a need to review the literature on what has been documented thus
far. In particular, we review the positive and associated negative properties of the plant. In doing so,
we specifically looked at the chemistry, medicinal and nutritional properties, as well as the ecological
implications of the plant. This review sought to collate the important research findings reported on
M. oleifera to date, enable researchers to identify the existing research gaps and allow the industry to
explore the collated information in developing new products.
2. Phytochemicals in Moringa oleifera
Plants are devoid of mobility to defend themselves from external stressful conditions, and
they have instead evolved and armed themselves with several secondary metabolites to counter
stress from temperature, water, light intensity, herbivory and microbial attack [17]. M. oleifera
was initially introduced to various communities with very little knowledge about its chemistry.
However, in recent years, various studies have reported the plant as a reliable source of potential
health-improving chemicals. As expected, the chemistry of M. oleifera is interesting, comprising of
different classes of compounds. Moreover, the plant has been shown to have advanced biosynthetic
pathways which ultimately result in a diversified chemical profile [18]. To date, various compounds
such as glucosinolates [19], flavonoids [18], phenolic acids [20], and other compounds found in
M. oleifera have been investigated.
2.1. Glucosinolates
Glucosinolates (GS) are a heterogeneous group of sulfur and nitrogen containing glycosidic
secondary metabolites [21,22]. GS are secondary compounds relatively unique to the Moringaceae
family [19,22] and to the family of Brassicaceae which include cabbage, broccoli and cauliflower. As a
group, these compounds are widely spread across different parts of the plant, with the seeds containing
the highest concentrations compared to the leaves [23]. These compounds are derived from amino acid
precursors and, as such, they can either comprise short- and long-chain aliphatic glucosinolates (Ile, Leu,
Val, Ala and Met), indolic glucosinolates (Trp) and aromatic glucosinolates (Tyr and Phe) [4,24,25].
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Interestingly, it has been shown that a certain type of the compounds is restricted in a certain parts
of the plant, for instance, benzyl glucosinolate (glucotropaeolin) is predominant in the roots [19],
whilst glucomoriginin is commonly found in stems, flowers, pods, leaves and seeds [26]. Apart
from the intra-plant variation, the levels of glucosinolates have been noted to vary between plants
collected from different geographical areas [19]. The most abundant GS molecule in M. oleifera is
glucomoriginin and its acylated derivative [20]. The acylated isomers of glucomoriginin represent an
interesting phenomenon since this could be regarded as an evolutionary strategy by the plant in order
to maximize its metabolite composition. This is a phenomenon which was seen with other metabolites
such as phenolic compounds in M. oleifera [20]. It was also observed that the three acylated isomers
of glucomoriginin elute at different chromatographic regions during reverse phase LC-MS analyses,
an indication that they differ in terms of polarity [27], which might have effects on the bioavailability.
In plants, it is believed that GS are biologically active, however, their metabolised products
(i.e., isothiocyanates, nitriles, thiocyanates, epithionitriles and oxazolidines) are deemed to be highly
active [28,29]. Elsewhere, oxidative stress has been shown to be a potent inducer of GS in plants [27]
and, by extension, GS molecules are believed to mitigate effects associated with oxidative stress.
As such, the use of GS molecules as direct and indirect antioxidants have been investigated [30].
Interestingly, GS molecules have been shown to possess anti-cancer activities [29]. Protective effects of
GS molecules against neurodegenerative diseases has been shown elsewhere [31].
2.2. Flavonoids
Similar to the GS molecules, flavonoids are highly active metabolites produced by plants [32].
Consumption of flavonoids has been linked with reduced risks associated with various diseases [33,34].
As such, humans prefer foods rich in these nutraceuticals since their consumption has been positively
correlated with the delayed onset of some age-related diseases [35]. Intake of flavonoids has also
been shown to have positive effects on cancer-related diseases [36] and as potent anti-inflammatory
agents [37]. Elsewhere, these molecules have been shown to have application in the cosmetic and skincare
industry [38,39]. M. oleifera, like any other plants, produces flavonoids and to date, the chemically
diverse profile of flavonoids has been reported in M. oleifera [18,40]. Only four types of flavonoids,
namely quercetin, kaempferol, isorhamnetin and apigenin, have been reported in the plant, and
myricetin has also been reported but with very little convincing analytical data [41]. The chemistry
of M. oleifera plant is interesting considering the chemical diversity of its flavonoids. Unlike other
plants, M. oleifera diversifies its flavonoids through complex glycosylation patterns. For instance,
when compared to its closely related species, M. ovalifolia, it was found that M. oleifera produces
similar aglycone flavonoids (quercetin, kaempferol and isorhamnetin), but the differences arise
when the flavonoid glycosides are concerned. Here, M. oleifera is shown to attach different types of
sugars on its flavonoid aglycones. Using quercetin as an example (Figure 1) it can be seen that this
flavonoid is glycosylated using various types of sugar attachment [18,40]. Interestingly, the same
type of glycosylation has been noted in another closely related species, M. stenopetala [19]. Some of
the sugar modification includes, amongst others, acetyl hexose, malonyl hexose, di-glycosylation,
and tri-acylation [40]. As seen with the GS molecules, the attachment of groups such as acetyl on
the sugar moiety of the active metabolites changes its polarity and ultimately its bio-availability.
Therefore, this type of diversification (through glycosylation) by M. oleifera is an indication that the
plant can be a useful source of bio-available flavonoid compounds which can be used in physiological
environments at different polarities. It is also important to mention that the same glycosylation
patterns have been noted with other similar flavonoids such as kaempferol and isorhamnetin [40].
This phenomenon of diversification can be said to be species/genus specific because other plants
such as Vernonia fastigiata diversify their flavonoid composition by swapping sugar positions [42].
For instance, V. fastigiata produces two isobaric molecules, quercetin-3-O-hexoside-O-pentoside and
quercetin-3-O-pentoside-O-hexoside, both appearing at m/z 595.1245 but at different retention times,
again indicating differences in polarities [42]. As such, the above is an indication that advanced
analytical techniques need to be developed in order to fully cover the flavonoid composition of the
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plant. In another example, a flavonoid peak appearing at m/z 593 with fragment ions at m/z 353 and 473
was once identified as Multiflorin B [40], which was later disputed and re-identified as Vicenin-2 [18],
a molecule with potential health benefits [43].
Figure 1. Different structures of Moringa oleifera chemical compounds, mainly quercetin glycosides
showing different glycosylation pattern. (A) quercetin-acetylhexose; (B) quercetin-rutinoside; (C)
quercetin-triacetylhexose; (D) quercetin-hydroxy-methylglutaroyl hexose; (E) quercetin-malonylhexose;
(F) quercetin-dihexose; and (G) quercetin-hexose.
Strangely, several studies have indicated that M. oleifera does not produce a highly bio-available
flavonoid called rutin. Research conducted on the two species of Moringa (M. stenopelata and M. oleifera),
discovered that, though the two plants are genetically related, M. stenopelata leaves contained rutin as
one of the predominately active compounds in amounts as much as 2.3% of dry leaf weight, which was
not found to be present in M. oleifera [44]. As indicated above, in a similar study by Makita et al. [18],
it was reported that M. oleifera contained more flavonoids than M. ovalifolia. However, on a close
look, all flavonoid compounds in M. ovalifolia were shown to be glycosylated only with rutinoside
sugar. From the two studies above, it was concluded that M. oleifera is incapable of glycosylating
its flavonoids with rutinoside sugar. However, in a twist of fate situation, in a follow-up study by
Makita et al. [45], comparing 12 cultivars of M. oleifera indicated that some of the cultivars contained
rutin and it was concluded that the presence of rutin is a cultivar-specific phenomenon, with 3 out of
12 cultivars of M. oleifera able to carry out this glycosylation. Therefore, rutinoside-bearing flavonoid
occurrence is cultivar-specific and as such, offers differences in pharmacological potency of plants.
This phenomenon might, however, result in negative perception towards M. oleifera as a nutraceuticals
source, as one has to be sure if the cultivar they are planting/processing contains rutin.
2.3. Phenolic Acids
Similar, to flavonoids, M. oleifera contains a large contingency of phenolic acid derivatives with
purported biological activities. From the leaf extracts, various isomers of chlorogenic acids (CGAs) have
been identified [40,45]. CGAs are ubiquitous and indispensable phenolic compounds found in various
plants [46], and are formed as a result of esterification between various forms of cinnamic acids and a
quinic acid molecule [45]. Due to the structural orientation (stereochemistry) of the quinic acid, different
isomers of CGAs can be formed and these differ from one plant to another [47] and to date, coffee beans
have been found to contain the largest composition of the compounds [48]. However, LC-MS based
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analyses of 80% methanolic extracts of M. oleifera leaves revealed that the plant contains structurally
diverse chlorogenic acids such as caffeoylquinic acids, feruroylquinic acids and coumaroylquinic
acids [45]. Each of these class of compounds was found as a group of isomers, for instance, more
than four peaks of caffeoylquinic acids appearing at m/z 353, represented as positional isomers and
geometrical isomers thereof. The former is believed to be formed enzymatically [46] whilst the latter are
believed to form as a result of UV exposure (in a form of sunlight), even though, elsewhere, they were
shown to form as a result of metabolic activity associated with a defensive mechanism of a plant [49].
Chlorogenic acids, in general, are known to offer protection against oxidative stress-related diseases.
To substantiate the above, the levels of different positional and geometrical isomers of CGAs were
found to be perturbed by oxidative stress induced by gamma radiation of M. oleifera leaves [20]. Such
findings gave an indication that geometrical isomers of this plant are not just mere structural artefact,
but biologically active compounds. According to Ramabulana et al. [20], the structurally diverse
composition of CGA molecules found in M. oleifera could be an evolutionary strategy to maximize
biologically active molecules through isomerization with an intention to increase its defensive chemical
arsenal against various stressors. This phenomenon demonstrates the “better be ready than sorry”
phenomenon which states that plants create a defensive environment by producing a large contingency
of structurally diverse defence compounds (phytoanticipins and phytoalexins) in order to strengthen
their innate immunity to be deployed against various forms of stress. Therefore, the constitutive
presence of CGA molecules in M. oleifera plants offers an overwhelming pharmacological advantage,
since these compounds have been associated with beneficial health attributes [48].
The amount of phenolic acid in M. oleifera varies depending on several factors, such as the rainfall
received. Water deficiency in plants results in oxidative stress and the plants, in turn, respond by
increasing production of antioxidant compounds [50]. This was confirmed by Leone et al. [51], who
observed that M. oleifera plants grown in water stressful environments had greater amounts of total
phenolics and antioxidant capacities. The plant part also plays a role, as leaves have higher amounts of
phenolics when compared to roots [52]. Other factors affecting phenolic acid concentration include
the harvesting stage of the plant, cultivar of M. oleifera and the extraction method used [53–55]. Free
hydroxyl compounds contained by the phenolics found in M oleifera leaves are responsible for reduction
reactions which aid in the prevention of degenerative diseases such as diabetes [56,57].
2.4. Vitamins and Minerals
Moringa oleifera has been described as the most nutritious tree yet discovered [58]. We summarise
some of the nutritional properties of the plant in Table 1.
Table 1. Properties of vitamins and minerals found in Moringa oleifera plant.
Bioactive
Compound
Specific compound
Properties
References
Vitamin A
Retinol, Retinal and Retinoic acids
Leaves contain 11,300–23,000 IU
(international unit) of vitamin A.
[5,59]
Vitamin B
Folates, such as
5-Formyl-5,6,7,8-tetrahydrofolic acid,
5,6,7,8-tetrahydrofolic acid and
5-Methyl-5,6,7,8-tetrahydrofolic acid
Involved in DNA synthesis and
cell division.
[60]
Carotenoids
β-carotene
Ranges from 6.63 mg/100 g in
fresh leaves to about 39.6 mg/100 g
in air-dried leaves.
[4,61,62]
Vitamin C
Ascorbic acid
Found in amounts of about 200
mg/100 g (greater than in orange
fruits). Acts as an antioxidant.
[63–65]
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Table 1. Cont.
Bioactive
Compound
Minerals
Specific compound
Properties
References
Potassium (K), Iron (Fe), Calcium (Ca)
and Magnesium (Mg).
Contains more calcium, iron and
potassium than in milk, spinach
and bananas respectively.
Vegetative parts and immature
fruits contain the most potassium.
[5,8,26]
2.5. Tannins
Tannins are water-soluble polymeric phenolics that bind to proteins and alkaloids [66]. The amount
of tannins in M. oleifera range between 13.2 g (tannin acid equivalent) TAE/kg and 20.6 g/kg in air-dried
leaves [67,68]. In leaves, they contribute to about 3.2% of dry matter [69]. However, the roots of the
M. oleifera have more of condensed tannins (proanthocyanidins) than leaves, which results in the
roots having a higher antioxidant activity [52]. This is because tannins are involved in the reduction
of peroxyl radicals due to hydroxyl groups (OH-) [70]. Furthermore, the tannins in M. oleifera have
been reported to contribute to anti-cancer, antimicrobial and anti-hepatoxic activity [55]. However,
in animals such as goats, tannins from trees such as Vachellia nilotica have been reported to reduce
feed intake, nutrient digestibility and nitrogen retention [71], because of the astringent taste and their
ability to precipitate proteins which renders them indigestible. Lu et al. [72] reported that inclusion of
M. oleifera leaf meal (≥10%) in poultry diets resulted in reduction in egg weight due to tannins causing
lower protein retention and digestibility. Therefore, some researchers have recommended less than
10% inclusion of M. oleifera in poultry diets, with no effects on the feed intake [73].
3. Nutritional Aspects of Moringa oleifera
3.1. In Humans
In addition to its abundant supply of bioactive phytochemicals that are important in ethnomedicinal
management of diseases, M. oleifera is also commonly utilised as a food crop, thus making it a functional
crop [74]. Due to its high drought and disease resistant properties, M. oleifera is often used as a
famine food in several African communities [75,76]. The use of M. oleifera as a food crop by humans is
supported by the fact that its leaves are an abundant source of polyunsaturated fatty acids (PUFAs) such
as omega-3 (ω-3) and omega-6 (ω-6) apart from the micro-elements and protein qualities [77], which
are important in vitalising the body and in cardiovascular function. M. oleifera pods and flowers have a
high content of total monounsaturated fatty acids while the seeds and oil from the seed possess a high
content of oleic and palmitoleic acid [78]. Oleic and palmitoleic acid are important in lowering plasma
cholesterol levels and ameliorating the effects of diabetes and insulin resistance [79]. Generally, the
different parts of M. oleifera possess low saturated fatty acid (SFAs), high monounsaturated fatty acids
(MUFA) and PUFA content that can be useful for human health, especially if food is supplemented and
fortified with M. oleifera.
As mentioned above, different parts of the M. oleifera plant are rich in mineral content
(micro-elements) such as potassium (K), iron (Fe), calcium (Ca) and magnesium (Mg) [80]. Human
consumption of M. oleifera can thus be beneficial in preventing negative health outcomes associated
with mineral deficiencies. Echoing the above, M. oleifera kernels are rich in proteins [68] and as
such, can be used as a good source of protein particularly for human food product formulation and
supplementation. Experimental proximate studies have also shown that the M. oleifera leaf powder
consists of carbohydrates and proteins [68] that can be used to increase the nutritional value of staple
foods fortified with the M. oleifera foliage. Inclusion of more than 1% weight for weight (w/w) of M.
oleifera leaf powder has shown to result in reduced acceptability due to bitterness associated with
the plant [81]. Despite its widespread potential as a nutritional supplement for human consumption,
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there is a need to perform further in vitro and in vivo experimental studies on the bioavailability and
digestibility of nutrients in M. oleifera.
3.2. In Livestock
Moringa oleifera is a multiple purpose tree that is used as a medicinal plant, spice, and food, among
other uses. In addition to its use in ethnomedicine, M. oleifera has several agricultural applications. It has
successfully been used as a fertiliser and a natural biopesticide against several plant pathogens [82].
Nutritional analyses of M. oleifera leaves and seeds have shown that they possess a high protein
content, carotenoids, minerals and vitamins [69]. The presence of high protein content and other
important nutrients [69,83] makes M. oleifera leaves an important contributor to livestock feed quality
and quantity [83]. Vitamins, in particular, contribute significantly to the immune systems of the animals
thus preventing the development of several diseases in the livestock [8]. The high nutritional value of
M. oleifera seeds and leaves makes it suitable for livestock feed supplementation to either improve
the growth performance of the livestock or replace traditional crops and provide an economically
sustainable source of feed [82]. Previous studies have shown that supplementing livestock with feed
containing M. oleifera leaves improves digestibility [84], and confers beneficial effects on growth and
carcass characteristics of animals whose diet is supplemented with M. oleifera [82].
Due to droughts and low rainfalls recorded in most tropical regions, supplying feed for livestock by
farmers has increasingly become a major challenge, particularly during dry seasons. In some southern
African regions that are characterised by harsh climatic conditions such as the Limpopo Province in
South Africa, smallholder farmers are being encouraged to cultivate M. oleifera to supplement their
livestock feed due its high nutritional value and its cash-earning potential [76]. The M. oleifera seed
also has a high oil content [85] and may be used for both livestock and human consumption.
The use of M. oleifera as a nutritional supplement in animal feed must be done with caution. Some
parts of the M. oleifera plants, such as the leaves and bark, have antinutritional factors and there is a
limit, not being in excess of more than 10% (w/w) of the diet [73].
4. Medicinal Properties of Moringa oleifera
As a medicinal plant duped the ‘miracle tree’, M. oleifera is used extensively and broadly in a
number of ailments, most of which have been tested pharmacologically and clinically in various
mechanistic and animal models. It is thus outside the scope of this review to exhaustively discuss this
extensive list of studies done on M. oleifera extracts, but we thus limit our discussion to highlighting a
selected few medicinal properties of M. oleifera extracts.
4.1. Antioxidant Properties
Extracts from the different solvents and plant parts of the M. oleifera are known to possess
antioxidant properties [86]. Leaf methanol and ethanol extracts, in particular, have shown some
scavenging properties towards superoxyl and peroxyl radicals [86,87]. Using a UV accelerated method
at 50 ◦ C, M. oleifera seed oil fraction was evaluated for their protection against rancidity to fresh
sunflower oil and demonstrated superior antioxidant properties. When these activities were compared
with those of α-tocopherol and BHT (common synthetic antioxidant agents) using the same method on
the same sunflower oil, M. oleifera seed oil fraction exhibited higher antioxidant activity than the two
known agents [88]. In another study, aqueous ethanolic extracts of M. oleifera leaf and flower, using
biochemical oxidative tissue markers, led to a significant decline in rat liver damage compared to the
control treatment [89]. The seed, fruit and leaf aqueous M. oleifera extracts were examined for their
potential to prevent DNA oxidative damage as well as their antioxidant properties. The results revealed
that these extracts have a significant potential of inhibiting DNA damage as well as synergistically
inhibit with trolox, in an effective sequence of leaf > fruit > seed [90].
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An experimental study on goats fed with leaf extracts of M. oleifera showed that treatment with
M. oleifera reduced lipid peroxidation and increased the antioxidant activity of glutathione peroxidase
and catalase [91]. Thiobarbituric acid reactive substance (TBARS) values were significantly lower in
chicken sausage samples incorporated with 0.5%, 0.75% and 1% M. oleifera leaf powder, compared to the
negative and the positive (BHT) controls throughout the five weeks storage duration at 4 ◦ C [92]. On the
other hand, Hazra et al. [93] reported a comparably lower TBA value of buffalo meat supplemented
with 1.5% M. oleifera leaf extract than those of the control treatment. Compared to the control, the leaf
extracts (0.1%) were shown to magnificently reduce lipid oxidation in cooked patties of goat meat [94].
Similar trends are also reported in recent research findings [95], where goat meat fed with meal
supplemented with Moringa oleifera leaf or sunflower cake (SC) or grass hay (GH) were compared for
their antioxidant properties. Moringa-supplemented meat had higher scavenging potential towards
ABTS (93.5%) and DPPH (59%) than the other two meal supplements. These results can be attributed
to the inhibition of lipid peroxidation by the antioxidant phytochemical agents found in M. oleifera
leaves such as polyphenols. Literature is replete with numerous other findings on the antioxidant
properties of M. oleifera extracts and thus cannot be overemphasised here. It is prudent to mention
that, of the most literature reviewed, phenolic acids and flavonoids feature prominently as responsible
agents for most of the reported antioxidant activities (Table 2).
4.2. Anti-Inflammatory Properties
Inflammation is one of the key characteristics of the diseases that result from the tilted balance
of anti-inflammatory cytokine regulated by T helper cells [96]. Type 2 diabetes, a result of metabolic
dysfunction, is linked to elevated levels of systemic pro-inflammation markers [97]. Diabetic
patients exhibit elevated levels of both TNF-α and IL6 contributing to the advancement of micro
and macrovascular changes, which is a characteristic of diabetic patients. The seeds and pods
of M. oleifera have been highlighted in numerous studies as having positive anti-inflammatory
properties [98–100]. The root extracts of M. oleifera were reported to have acute anti-inflammatory
properties in a carrageenin-induced rat paw oedema test [101,102]. An evaluation of the stem bark
extracts for their immunomodulation properties on human monocyte cells (THP-1) revealed substantial
inhibition of pro-inflammation cytokines (TNF-α, IL-6, and IL-1β), as well as reactive oxygen species
(ROS) and nitric oxide (NO) production [103]. Eicosane, cis-13-octadecenoic acid, hexadecane, benzoic
acid, n-hexadecanoic acid, heptadecane, dodecane, hexadecanoic acid, methyl ester, β-sitosterol and
ethyl ester are some of the metabolites identified in root, leaf and seeds of M. oleifera [4,104,105], and
some of which are known for their anti-inflammatory properties [106–108]. The anti-inflammatory
properties of M. oleifera confirm the ethnomedicinal uses of the plant to treat various diseases that are
associated with inflammatory processes.
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Table 2. Some reported antioxidant properties of different plant parts of M. oleifera.
Antioxidant Model Used
Candidate Compounds
Solvent
Ref.
Leaf
1,1-diphenyl 2-picrylhydrazyl (DPPH)
β-carotene-linoleic acid
Superoxide radical scavenging
Liposome Peroxidation
Enzymatic Lipid Peroxidation of Microsomes
Induced by NADPH/ADP/Fe3+
Linoleic Acid Peroxidation System
Superoxide dismutase (SOD)
Catalase
Glutathione peroxidase
Nitric oxide (NO) radical scavenging
2,2′ -azino-bis-3-ethylbenzothiazoline-6-sulphonic
acid (ABTS)
Ferric Reducing Iron Power (FRAP)
Crude extracts, quercetin, kaempferol, gallic, chlorogenic,
ellagic, ferulic acid, rutin, gallic acid, vanillin
Quercetin, kaempferol, gallic, chlorogenic, ellagic, ferulic
acid, rutin, gallic acid, vanillin
Quercetin, kaempferol
Quercetin, kaempferol
Crude extracts, quercetin, kaempferol, gallic, chlorogenic,
ellagic, ferulic acid, rutin
Quercetin, kaempferol
Crude extracts, quercetin, kaempferol, gallic, chlorogenic,
ellagic, ferulic acid, rutin
Crude extracts, quercetin, kaempferol, gallic, chlorogenic,
ellagic, ferulic acid, rutin
Crude extracts
Crude extracts
Water, 70% ethnol, 80% ethanol, 80%
methanol. Chloroform, acetone
Water, 70% ethnol, 80% methanol,
chloroform
Water, 70% ethnol, 80% methanol
Water, 70% ethnol, 80% methanol,
Water, 70% ethnol, 80% methanol,
acetone, chloroform
Water, 70% ethnol, 80% methanol
[80,82,84–87,89,90]
[80,82,85]
[80]
[80]
[80,82,86]
[80]
Chloroform, water, 80% ethanol, acetone
[82,84,86,90]
Chloroform, water, 80% ethanol, acetone
[82,84,86,90]
Water, acetone
Water, acetone
[86,90]
[86]
Crude extracts
Water, acetone
[86]
Crude extract
Water, 80% ethanol, acetone
[85,86]
Chloroform/methanol (1:1), diethylether,
n-butanol, and water
[83]
Water, 80% ethanol
[84,85]
Water, 80% ethanol
[84,85]
80% ethanol
80% ethanol
[84]
[84]
Water
[85]
Water
[85,88,90]
Seed
UV accelerated method
DPPH
FRAP
SOD
Catalase
β-carotene-linoleic acid
Lipid Peroxidation
Crude extract fractions
Crude extract, gallic acid, chlorogenic acid, ellagic acid,
ferulic acid, kaempferol, quercetin, vanillin.
Crude extract, gallic acid, chlorogenic acid, ellagic acid,
ferulic acid, kaempferol, quercetin, vanillin
Crude extract
Crude extract
gallic acid, chlorogenic acid, ellagic acid, ferulic acid,
kaempferol, quercetin, vanillin
Crude extracts, gallic acid, chlorogenic acid, ellagic acid,
ferulic acid, kaempferol, quercetin, vanillin
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Table 2. Cont.
Antioxidant Model Used
Candidate Compounds
Solvent
Ref.
80% ethanol
80% ethanol
80% ethanol
80% ethanol
[84]
[84]
[84]
[84]
Water, 80% ethanol
[84,85]
Water, 80% ethanol
[84,85]
80% ethanol
80% ethanol
[84]
[84]
Water
[85]
Water
[85]
80% ethanol
80% ethanol
80% ethanol
80% ethanol
[84]
[84]
[84]
[84]
Flower
DPPH
FRAP
Superoxide dismutase (SOD)
Catalase
Crude extract
Crude extract
Crude extract
Crude extract
Pod
DPPH
FRAP
SOD
Catalase
β-carotene-linoleic acid
Lipid Peroxidation
Crude extract, gallic acid, chlorogenic acid, ellagic acid,
ferulic acid, kaempferol, quercetin, vanillin
Crude extract, gallic acid, chlorogenic acid, ellagic acid,
ferulic acid, kaempferol, quercetin, vanillin
Crude extract
Crude extract
Gallic acid, chlorogenic acid, ellagic acid, ferulic acid,
kaempferol, quercetin, vanillin
Gallic acid, chlorogenic acid, ellagic acid, ferulic acid,
kaempferol, quercetin, vanillin
Stem
DPPH
FRAP
SOD
Catalase
Crude extract
Crude extract
Crude extract
Crude extract
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4.3. Anti-Diabetic Properties
Metabolic syndrome (MetS) comprises of a cluster of risk factors that are related to glucose and
lipid metabolism (obesity) as well as cardiovascular dysfunction (blood pressure) [109,110]. Recently,
the prevalence of MetS health outcomes such as Type 2 diabetes are on the rise, posing a public health
burden particularly in the developing world [111]. This has necessitated the need to explore the
therapeutic efficacy of alternative and complementary treatments.
Experimental animal models reveal that orally administered M. oleifera leaf extract reduces
the progression of fructose-induced diabetes [112]. Later studies show that M. oleifera leaf powder
ameliorates alloxan-induced hyperglycemia [113], indicating its potential in managing diabetes.
These anti-diabetic properties of M. oleifera were further demonstrated in an animal study that
revealed aqueous leaf extracts normalising diet- and streptozotocin-induced hyperglycaemia and
hyperinsulinaemia [114,115]. Jaiswal et al. [116] assessed the variable doses of the aqueous M. oleifera
leaf extract on their anti-diabetic potential on mildly- and severely-induced diabetic rats. Decreased
levels of glucose (29.9%) were recorded from normal rats administered with 200 mg/kg of M. oleifera.
In cases of severely diabetic rats, glucose levels were brought to near normal levels with a reduction of
69.2% and 51.2%. An accompanying improvement in total protein and haemoglobin levels was also
reported after 21 days of treatment with M. oleifera and thus favourably reducing diabetes [116–118].
The hypoglycemic effect of M. oleifera extract in this study was found to be comparably similar to
Glipizide (an anti-diabetic drug). This experimental evidence further attests to the purported potential
of M. oleifera extracts in managing diabetic conditions. N-Benzyl nitriles, benzyl thiocarbamates,
a benzyl ester and N-benzyl carbamates from the fruit powder extract of M. oleifera were reported
to have expressively stimulated insulin generation in the beta cells of rodent pancreas. The released
insulin had lipid peroxidation and cyclooxygenase enzyme inhibitory properties [119].
Hyperglycaemia emanating from either insulin action orbits abnormal production results in
renal, cardiovascular, to ocular complications [117]. This prompted the use of natural medicines in
the management of diabetes [118]. For example, the use of M. oleifera aqueous leaf extract over a
2-month period re-established all the changes (body weight, plasma glucose, insulin and lipid profile)
to normal/near normal on Type 1 diabetic rats [114]. A study by Divi et al. [114] reports M. oleifera
aqueous leaf extracts to have potent antihyperlipedemic and antihyperglycemic properties on Type
1 and Type 2 diabetic rats. When the anti-diabetic effects of aqueous extract of M. oleifera leaves
were analysed in histomorphometrical, ultrastructural and biochemical studies by Yassa et al. [120],
the altered FPG levels were reduced more than 2-fold and lowered malondialdehyde (>3-fold) and
glutathione (>3-fold). The damage to the islet cells was also reported to be reversed significantly.
The extract also led to a significant increase (31%) in the area with purple-modified stained β-cells
while decreasing (79%) the percentage area of collagen fibres in comparison to the control. Comparable
results were also reported by [121]. The major contributors to the progressive development and
complications of diabetes were that the weakened antioxidant defence systems prolonged oxidative
stress, as well as lipid peroxidation [122].
Improved glucose tolerance through the use of M. oleifera supplementation over extended periods
has been reported [123]. Gupta et al. [124] provide some plausible meaning and explanation to these
results when they discovered bioflavonoids in M. oleifera, which plays a crucial role in the uptake of
glucose in marginal tissues as well as regulating carbohydrate metabolism. The constituent metabolites
in M. oleifera enhance the secretion of insulin from β-cells. Bernal-Mizrachi et al. [125] report a marked
decline in immune-stained β-cells in diabetic rats. β-cells are the most abundant cells of the endocrine
pancreas according to Ross et al. [126], and it is the site where biochemical and histological changes
occur during short term treatments with M. oleifera leaf extracts [116,127–129].
4.4. Anti-Cancer Properties
Despite the progress made in the development of chemotherapy in treating cancer, adverse effects
such as skin irritation, nausea, nephrotoxicity, infertility, anaemia, and hair loss still exist [130]. It is for
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this reason that the natural plant-derived anti-cancer sources of treatment with limited side effects
are critical in the search for alternative cancer treatments. The potential of M. oleifera extracts to treat
cancer have previously been demonstrated [131–133]. In lung cancer cells, the aqueous fraction of
M. oleifera leaf extract is reported to have induced an apoptotic effect on HepG2 cells [134]. Leaf
extracts administered orally led to a significant reduction (52%) in the proliferation of HepG2 cells and
lung cancer cells [135]. One of the advantages of oral cancer therapy is that it leads to the prolonged
exposure of the cancer cells and the surrounding environment to the cytotoxic agents.
A thiocarbamate, niaziminin, derived from M. oleifera leaf, is structurally strict for the inhibition of
tumour-promoter-induced Epstein-Barr virus (EBV) activation [131]. Studies on the structure-activity
relationship showed that an acetoxy group at the 4’-position of niaziminin is an indispensable property
for tumour inhibition [136]. A related study by Jung [135], revealed that the aqueous M. oleifera leaf
extract (300 µg/mL) markedly decreased tumour cell growth, reduced internal ROS level as well as
inducing apoptosis in lung and other cancer cell types. Furthermore, M. oleifera extracts led to the
down-regulation of 90% of the tested genes by margins greater than 2-fold in comparison with the
non-treated cells. The authors concluded that the down-regulation of these genes was due to abnormal
RNA as a result of M. oleifera leaf extract treatment. Vasanth et al. [137] reported M. oleifera stem
bark extract facilitated silver nanoparticles (AgNPs) as exhibiting exceptional anti-cancer properties
on HeLa cells. These AgNPs are reported to exert their activity by increasing ROS production and
subsequently inducing apoptotic effect through inhibition of cell replication.
5. Side Effects of Moringa oleifera
Although more benefits of using M. oleifera for medicinal purposes overshadow its known harmful
effects, there are suggestions that it cannot be used in combination with other modern medicines in
humans. For example, anecdotal evidence suggests that when treating thyroids, M. oleifera compounds
in the leaf may aid thyroid function [138]. This evidence further suggests that it can possibly conflict
with other thyroid medication triggering drug interaction. It is perceived that M. oleifera could adversely
slow down the breaking down of substances in the liver [139–141]. In that regard, M. oleifera could
reduce the process of breaking down some medication in the liver. This could progress to cirrhosis
and liver failure resulting in malnutrition and weight loss, as well as decreased cognitive function.
In addition, M. oleifera has been noted to be a good regulator of insulin [142]. Therefore, patients
suffering from lack of insulin are bound to have adverse reductions in their sugar levels when using M.
oleifera for medicinal purposes [141,142]. It is hypothesised that it could decrease the blood sugar to
even lower levels when used in combination with other modern medications [141].
A study by Barichella et al. [143] assessed the use, acceptability and safety of M. oleifera on children
in Zambia. With regards to safety concerns, supplementation of 14 g per day of M. oleifera powder was
deemed safe for children and adolescents both in the short and long term. Barichella et al. [144] also
noted that mild nausea was reported in 20% of the children at various age groups when meals were
supplemented with 20 g of M. oleifera daily. These side effects were deemed acceptable by the Ethics
Committee [143]. Overall, the findings of this study underscore the fact that despite the lack of safety
information on the utility of M. oleifera, there are no scientifically proven side effects of M. oleifera to
this date [144].
6. Contraindications of M. oleifera
Despite the numerous positive possibilities associated with M. oleifera phytochemicals, there are
suspicions that it contains harmful substances [22,145,146]. M. oleifera contains harmful chemicals
such as alkaloids and other phytotoxins, which when consumed in high doses have potentially
nerve-paralysing properties and other adverse effects [146]. Some of these phytochemicals include
moringine, moringinine, estrogene, pectinesterase and phenols including tannin [22,145]. There are
also unconfirmed reports that M. oleifera stems and roots potentially contain harmful phytochemical
constituents, especially to pregnant women. Specifically, it is suspected that these elements of M. oleifera
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contain phytochemicals which have a potential of facilitating uterus contraction, leading to miscarriages
in pregnant women. It is also suspected that it has the ability to prevent implantation in women,
hence it has to be avoided by those attempting to conceive [147]. Some scientists suspect that the
extracts from the roots have a potential of even causing paralysis and death. However, it is important
to note that there are no major harmful effects of M. oleifera on humans that have been put forth by
the scientific community to this date [144]. Based on the studies, and ongoing research, that has been
conducted to date on both humans and animals, no adverse effects have been noted from M. oleifera
products [144,148]. Although research is still ongoing, currently there are no scientifically confirmed
toxic and harmful effects of M. oleifera extracts and products on both humans and animals.
7. Water Purification
People living in developing and underdeveloped countries drink highly contaminated water [136].
It is estimated that at least 15% of the world’s population lacks safe/clean drinking water. It is estimated
that water-related diseases kill more than 5 million people per annum globally [149]. The high cost of
chemicals used to treat water has led most people in the rural communities of developing countries to
rely on easily available and accessible water sources. Most of these sources are usually contaminated
and are also suggested to contain waterborne diseases [150]. Thus the use of M. oleifera seeds, which
are edible, as natural coagulants are now highly recommended because naturally occurring coagulants
are suggested to be safe for human health [150,151].
Moringa oleifera seeds have been reported to contain coagulation properties [150,151], which
are particularly recommended to use for high-turbid water (water with high levels of haziness or
cloudiness) [136]. However, the coagulation activity on M. oleifera has been found to be low for
low-turbid water [152,153]. When its seeds are dried, scarified, crushed and added to water, its powder
acts as a coagulant which binds the microscopic colloidal particles and bacteria to form clump
particles [154]. These particles settle at the bottom and the purified supernatant can be poured off [154].
Water treatment ranges from 2 seeds per 1 litre to 1 seed per 4 litres depending on the turbidity of the
water [152]. M. oleifera seeds contain 1% active polyelectrolyte that neutralizes the negatively charged
colloid mixture in the contaminated water [155]. There is a reduction in water conductivity, turbidity
and total solids in treated water [156]. Ndhlala et al. [55] attribute the positive effects of M. oleifera
through its antimicrobial properties against Klebsiella pneumoniae, Staphylococcus aureus bacteria and
Candida albicans fungus. Furthermore, M. oleifera seed has been suggested to remove about 90–99% of
the bacteria found in contaminated water [150].
While the use of M. oleifera in household water treatment is evident, its use of on a large scale is
not detailed. Instead, in large scale water treatment plants, aluminium sulphate and potash are more
commonly used as conventional chemical coagulants. Additionally, there have been suggestions of a
secondary increase of bacteria after water coagulation, as well as the purified water containing some
pathogenic germs or microorganisms [157]. In addition, the coagulant from M. oleifera is not available
in pure or preserved form as it should be prepared fresh [157]. This limits its use and accessibility
in areas where M. oleifera is not grown. M. oleifera increases the levels of organic matter in treated
water, which may offset the colour, taste and odour of water, and these problems have the potential to
worsen when treated water is stored for longer periods [153]. Due to the lack of adequate literature,
we recommend further research on the toxicity in water purification used for human consumption,
with possible implications for their large scale use.
8. Invasiveness and Allelopathy of Moringa oleifera
The coexistence of competitive invasive vegetation with native plants is crucial towards the
long-term sustainable production of ecosystems [158]. Plants which are considered easily adaptable,
as well as moderately invasive, have a high potential to impact the stability of ecosystems and their
production of ecosystem services. For example, M. oleifera generally grows in most soil types, except for
clayey soils, and grows well in harsh conditions in semi-arid and arid regions [159,160]. Consequently,
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there is a great concern that such a plant that easily grows in a wide range of conditions has the high
potential to effortlessly become invasive [161]. There are conflicting reports about the extent of the
invasiveness of M. oleifera, as it is regarded as potentially or moderately invasive, especially in the
tropics [162], as it has growth attributes of forming dense thickets around the parent plant [163].
The Invasive Species Compendium (CABI) classifies M. oleifera as a low potential invasive
species [164]. Comparable to other plant species with similar ecological growth attributes, it has
been suggested to cause dire ecological consequences in the wet/dry tropics around the world [164].
For example, in northern Australia, it has escaped from gardens, leading to it being considered a
minor weed in that region [163]. However, it may not necessarily be considered a problem plant in
agricultural areas, as it colonises river banks because of the high water table all year round [163].
A study conducted in Trinidad and Tobago, which tested the invasiveness of M. oleifera using the
Australian Weed Risk Assessment (WRA), concluded that the plant is a low-risk plant [155]. Instead,
this study led to it being classified as a bioenergy crop in the Caribbean [161]. As a result of such
conclusions, M. oleifera has been described as a naturalised plant crop in most of the countries in the
tropics and subtropics [4].
Due to its growth characteristics and adaptability, there are contradicting results from the use of its
extracts when investigating its potential to suppress or promote the growth of other vegetation. Some
studies have reported positive effects of other plants grown after spraying leaf extracts from M. oleifera.
For example, Fuglie [165], Mehboob et al. [166], Soliman et al. [167], Iqbal [168] and Nouman et al. [169]
all reported positive effects of extracts from M. oleifera on plant growth characteristics of different crops.
In Zambia, M. oleifera leaf extracts did not affect the time taken to germination of maize and wheat [170].
However, the leaf extract enhanced germination of sorghum resulted in delayed germination of
wheat [170]. Some negative effects of extracts from M. oleifera plant parts have also been reported in
parts of the world for both field and lab experiments. For instance, M. oleifera leaf extracts impeded the
rate of germination of mungbean (Vigna radiata (L) Wilczek) under laboratory conditions, while root
extracts also impeded its growth and yield under pot conditions in Bangladesh [171].
Elsewhere in Iraq, M. oleifera leaf, flower and seed extracts had negative effects on the seed
germination, shoot and root growth of wild mustard (Sinapis arvensis) plants but were stimulatory to
seed germination and growth of wheat (Triticum aestivum L.) seedlings [172]. In addition, M. oleifera
leaves showed negative allelopathic effects on faba bean (Vicia faba L) growth in Saudi Arabia [167].
Several allelochemicals have also been found within different parts of the M. oleifera plant. For example,
there are suggestions that the seeds and bark contain 4-(R-L-rhamnopyranosyloxy)-benzylglucosinolate
while, 4-(R-L-rhamnopyranosyloxy)-benzylglucosinolate and benzyl glucosinolate have been isolated
in M. oleifera roots [173]. However, the positive effects of the leaf extracts have been attributed to the
rich naturally occurring cytokinin, along with phytohormones and inorganic salts that are in a naturally
balanced concentration, as well as 4-(R-Lrhamnopyranosyloxy)-benzylglucosinolate, 3-caffeoylquinic
acid M. oleifera leaves reportedly contain [173]. These combinations increase the yield of crops when
applied exogenously [174].
The contradictory scientific publications regarding both the negative and positive effects of
extracts from M. oleifera on co-existing vegetation make it difficult to certify it as a fully invasive plant.
We came across no other records which classify M. oleifera as moderately or potentially invasive, except
the already cited report in Australia [162]. Most studies on extracts from different M. oleifera plant
parts (roots, leaves and stem) generally report positive effects on plant growth characteristics, and a
low proportion reports negative effects. Therefore, introducing this species, especially in degraded
ecosystems, should be done with care to avoid it colonizing native vegetation territory, since it easily
adapts to any growth conditions.
9. Conclusions
This work sought to review the beneficial and adverse properties of M. oleifera. Specifically,
the paper assessed the medicinal and nutritional properties and the ecological impact of the plant.
Plants 2019, 8, 510
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Grounded in the findings of this work, after meticulously interrogating a plethora of studies, we
conclude that:
•
•
•
•
•
•
•
Gram on gram, M. oleifera contains higher amounts of elemental nutrients than most
conventional vegetable sources which makes it a potentially lucrative crop to combat food
and nutritional insecurity.
There are no scientifically proven side effects of M. oleifera to this date, despite the lack of safety
information on its utility, particularly in humans.
Based on available M. oleifera, it produces a chemically diverse range of phytochemicals which
can be exploited for the development of pharmaceutical agents.
Due to a pool of phytochemicals found in M. oleifera extracts, a number of medicinal properties
have been reported to date.
M. oleifera holds great potential both as a food supplement and medicine, however, more clinical
trials are needed for the development of pharmaceutical agents.
M. oleifera has shown some potential as a water treatment agent and can be a useful resource
particularly in resource poor communities.
There is no literature that suggests that M. oleifera could be an invasive plant species, although
extreme caution has to be exercised when replanting, or introducing it, particularly in
degraded lands.
Author Contributions: T.T., B.N. and A.R.N. conceptualized the paper. T.T., B.N., N.E.M., T.T.N., H.P.M., M.S.
and A.R.N. wrote and approved the final version of the manuscript.
Funding: This study was funded by the Department of Science and Technology—Indigenous Knowledge System
-based Tech Innovation, Pretoria, and the National Research Foundation (NRF) South Africa.
Conflicts of Interest: The authors declare no conflicts of interest.
References
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
Ekor, M. The growing use of herbal medicines: Issues relating to adverse reactions and challenges in
monitoring safety. Front. Pharmacol. 2014, 4, 177. [CrossRef]
Bennett, R.N.; Wallsgrove, R.M. Secondary metabolites in plant defence mechanisms. New Phytol. 1994, 127,
617–633. [CrossRef]
Ma, Z.F.; Ahmad, J.; Zhang, H.; Khan, I.; Muhammad, S. Evaluation of phytochemical and medicinal
properties of Moringa (Moringa oleifera) as a potential functional food. S. Afr. J. Bot. 2019. (In press) [CrossRef]
Leone, A.; Spada, A.; Battezzati, A.; Schiraldi, A.; Aristil, J.; Bertoli, S. Cultivation, Genetic,
Ethnopharmacology, Phytochemistry and Pharmacology of Moringa oleifera Leaves: An Overview. Int. J.
Mol. Sci. 2015, 16, 12791–12835. [CrossRef]
Fahey, J.W. Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic and prophylactic
properties. Trees Life J. 2005, 1, 5.
Folkard, G.K.; Sutherland, J.P. Moringa oleifera: A tree and a litany of Potential. Agrofor. Today 1996, 8, 5–8.
Sharma, V.; Paliwal, R.; Sharma, P.; Sharma, S. Phytochemical analysis and evaluation of antioxidant activities
of hydro-ethanolic extract of Moringa oleifera Lam. pods. J. Pharm. Res. 2011, 4, 554–557.
Gopalakrishnan, L.; Doriya, K.; Kumar, D.S. Moringa oleifera: A review on nutritive importance and its
medicinal application. Food Sci. Hum. Wellness 2016, 5, 49–56. [CrossRef]
Shih, M.-C.; Chang, C.-M.; Kang, S.-M.; Tsai, M.-L. Effect of Different Parts (Leaf, Stem and Stalk) and Seasons
(Summer and Winter) on the Chemical Compositions and Antioxidant Activity of Moringa oleifera. Int. J.
Mol. Sci. 2011, 12, 6077–6088. [CrossRef] [PubMed]
Ashfaq, M.; Basra, S.M.A.; Ashfaq, U. Moringa: A miracle plant of agroforestry. J. Agric. Soc. Sci. 2012, 8,
115–122.
Daba, M. Miracle Tree: A Review on Multi-purposes of Moringa oleifera and Its Implication for Climate
Change Mitigation. J. Earth Sci Clim. Chang. 2016, 7, 366. [CrossRef]
Plants 2019, 8, 510
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
16 of 23
Anwar, F.; Latif, S.; Ashraf, M.; Gilani, A.H. Moringa oleifera: A food plant with multiple medicinal uses.
Phytother. Res. 2007, 21, 17–25. [CrossRef] [PubMed]
Ganguly, S. Indian ayurvedic and traditional medicinal implications of indigenously available plants, herbs
and fruits: A review. Int. J. Res. Ayurveda Pharm. 2013, 4, 623–625. [CrossRef]
Kumar, S.; Gopal, K. Screening of plant species for inhibition of bacterial population of raw water. J. Environ.
Sci. Health A Tox. Hazard. Subst. Environ. Eng. 1999, 34, 975–987. [CrossRef]
Mofijur, M.; Masjuki, H.H.; Kalam, M.A.; Atabani, A.E.; Fattah, I.M.R.; Mobarak, H.M. Comparative
evaluation of performance and emission characteristics of Moringa oleifera and Palm oil based biodiesel in a
diesel engine. Ind. Crops Prod. 2014, 53, 78–84. [CrossRef]
Karthy, E.S.; Ranjitha, P.; Mohankumar, A. Antimicrobial potential of plant seed extracts against Multidrug
Resistant Methicillin Resistant Staphylococcus aureus (MDR—MRSA). Int. J. Biol. Sci. 2009, 1, 34–40. [CrossRef]
Ncube, B.; Finnie, J.F.; Van Staden, J. Quality from the field: The impact of environmental factors as quality
determinants in medicinal plants. S. Afr. J. Bot. 2012, 82, 11–20. [CrossRef]
Makita, C.; Chimuka, L.; Steenkamp, P.; Cukrowska, E.; Madala, E. Comparative analyses of flavonoid
content in Moringa oleifera and Moringa ovalifolia with the aid of UHPLC-qTOF-MS fingerprinting. S. Afr.
J. Bot. 2016, 105, 116–122. [CrossRef]
Bennett, R.N.; Mellon, F.A.; Foidl, N.; Pratt, J.H.; DuPont, M.S.; Perkins, L.; Kroon, P.A. Profiling glucosinolates
and phenolics in vegetative and reproductive tissues of the multi-purpose trees Moringa oleifera L. (Horseradish
tree) and Moringa stenopetala L. J. Agric. Food Chem. 2003, 51, 3546–3553. [CrossRef]
Ramabulana, T.; Mavunda, R.D.; Steenkamp, P.A.; Piater, L.A.; Dubery, I.A.; Madala, N.E. Perturbation of
pharmacologically relevant polyphenolic compounds in Moringa oleifera against photo-oxidative damages
imposed by gamma radiation. J. Photochem. Photobiol. B Biol. 2016, 156, 79–86. [CrossRef]
Mithen, R. Glucosinolates-biochemistry, genetics and biological activity. Plant Growth Regul. 2001, 34, 91–103.
[CrossRef]
Fahey, J.W.; Zalcmann, A.T.; Talalay, P. The chemical diversity and distribution of glucosinolates and
isothiocyanates among plants. Phytochemistry 2001, 56, 5–51. [CrossRef]
Maldini, M.; Maksoud, S.A.; Natella, F.; Montoro, P.; Petretto, G.L.; Foddai, M.; De Nicola, G.R.; Chessa, M.;
Pintore, G. Moringa oleifera: Study of phenolics and glucosinolates by mass spectrometry. J. Mass Spectrom.
2014, 49, 900–910. [CrossRef]
Brown, P.D.; Tokuhisa, J.G.; Reichelt, M.; Gershenzon, J. Variation of glucosinolate accumulation among
different organs and developmental stages of Arabidopsis thaliana. Phytochemistry 2003, 62, 471–481. [CrossRef]
Clarke, D.B. Glucosinolates, structures and analysis in food. Anal. Chem. 2010, 9660, 310–325. [CrossRef]
Amaglo, N.K.; Bennett, R.N.; Lo Curto, R.B.; Rosa, E.A.S.; Lo Turco, V.; Giuffrida, A.; Lo Curto, A.; Crea, F.;
Timpo, G.M. Profiling selected phytochemicals and nutrients in different tissues of the multipurpose tree
Moringa oleifera L., grown in Ghana. Food Chem. 2010, 122, 1047–1054. [CrossRef]
Ramabulana, T.; Mavunda, R.D.; Steenkamp, P.A.; Piater, L.A.; Dubery, I.A.; Ndhlala, A.R.; Madala, N.E.
Gamma radiation treatment activates glucomoringin synthesis in Moringa oleifera. Rev. Bras. Farmacogn.
2017, 27, 569–575. [CrossRef]
Bones, A.M.; Rossiter, J.T. The enzymic and chemically induced decomposition of glucosinolates.
Phytochemistry 2006, 67, 1053–1067. [CrossRef]
Brunelli, D.; Tavecchio, M.; Falcioni, C.; Frapolli, R.; Erba, E.; Iori, R.; Rollin, P.; Barillari, J.; Manzotti, C.;
Morazzoni, P.; et al. The isothiocyanate produced from glucomoringin inhibits NF-kB and reduces myeloma
growth in nude mice in vivo. Biochem. Pharmacol. 2010, 79, 1141–1148. [CrossRef]
Tumer, T.B.; Rojas-Silva, P.; Poulev, A.; Raskin, I.; Waterman, C. Direct and indirect antioxidant activity of
polyphenol- and isothiocyanate-enriched fractions from moringa oleifera. J. Agric. Food Chem. 2015, 63,
1505–1513. [CrossRef]
Jaafaru, M.S.; Nordin, N.; Shaari, K.; Rosli, R.; Abdull Razis, A.F. Isothiocyanate from Moringa oleifera seeds
mitigates hydrogen peroxide-induced cytotoxicity and preserved morphological features of human neuronal
cells. PLoS ONE 2018, 13, e0196403. [CrossRef] [PubMed]
Cook, N.C.; Samman, S. Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources.
J. Nutr. Biochem. 1996, 7, 66–76. [CrossRef]
Plants 2019, 8, 510
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
17 of 23
Wedick, N.M.; Pan, A.; Cassidy, A.; Rimm, E.B.; Sampson, L.; Rosner, B.; Willett, W.; Hu, F.B.; Sun, Q.; van
Dam, R.M. Dietary flavonoid intakes and risk of type 2 diabetes in US men and women. Am. J. Clin. Nutr.
2012, 95, 925–933. [CrossRef] [PubMed]
Yao, L.H.; Jiang, Y.M.; Shi, J.; Tomas-Barberan, F.A.; Datta, N.; Singanusong, R.; Chen, S.S. Flavonoids in food
and their health benefits. Plant Foods Hum. Nutr. 2004, 59, 113–122. [CrossRef]
Prasain, J.K.; Carlson, S.H.; Wyss, J.M. Flavonoids and age-related disease: Risk, benefits and critical
windows. Maturitas 2010, 66, 163–171. [CrossRef] [PubMed]
Chen, A.Y.; Chen, Y.C. A review of the dietary flavonoid, kaempferol on human health and cancer
chemoprevention. Food Chem. 2013, 15, 2099–2107. [CrossRef] [PubMed]
Antunes-Ricardo, M.; Gutiérrez-Uribe, J.A.; Martínez-Vitela, C.; Serna-Saldívar, S.O. Topical anti-inflammatory
effects of isorhamnetin glycosides isolated from Opuntia ficus-indica. Biomed. Res. Int. 2015, 2015, 847320.
[CrossRef]
Allemann, I.B.; Baumann, L. Botanicals in skin care products. Int. J. Dermatol. 2009, 48, 923–934. [CrossRef]
Lai, J.S.; Lin, C.; Chiang, T.M. Tyrosinase inhibitory activity and thermostability of the flavonoid complex
from Sophora japonica L (Fabaceae) Trop. J. Pharm. Res. 2014, 13, 243–247.
Rodriguez-Perez, C.; Quirantes-Pine, R.; Fernandez-Gutierrez, A.; Segura-Carretero, A. Optimization of
extraction method to obtain a phenolic compounds-rich extract Moringa oleifera Lam leaves. Ind. Crops Prod.
2015, 66, 246–254. [CrossRef]
Matshediso, P.G.; Cukrowska, E.; Chimuka, L. Development of pressurised hot water extraction (PHWE) for
essential compounds from Moringa oleifera leaf extracts. Food Chem. 2015, 172, 423–427. [CrossRef]
Masike, K.; Khoza, B.S.; Steenkamp, P.A.; Smit, E.; Dubery, I.A.; Madala, N.E. A metabolomics-guided
exploration of the phytochemical constituents of Vernonia fastigiata with the aid of pressurized hot water
extraction and liquid chromatography-mass spectrometry. Molecules 2017, 22, 1200. [CrossRef]
Nagaprashantha, L.D.; Vatsyayan, R.; Singhal, J.; Fast, S.; Roby, R.; Awasthi, S.; Singhal, S.S. Anti-cancer
effects of novel flavonoid vicenin-2 as a single agent and in synergistic combination with docetaxel in prostate
cancer. Biochem. Pharmacol. 2011, 82, 1100–1109. [CrossRef]
Habtemariam, S. The African Moringa is to change the lives of millions in Ethiopia and far beyond. Asian Pac.
J. Trop. Biomed. 2016, 6, 355–356. [CrossRef]
Makita, C.; Chimuka, L.; Cukrowska, E.; Steenkamp, P.A.; Kandawa-Schutz, M.; Ndhlala, A.R.; Madala, N.E.
UPLC-qTOF-MS profiling of pharmacologically important chlorogenic acids and associated glycosides in
Moringa ovalifolia leaf extracts. S. Afr. J. Bot. 2017, 108, 193–199. [CrossRef]
Ncube, E.N.; Mhlongo, M.I.; Piater, L.A.; Steenkamp, P.A.; Dubery, I.A.; Madala, N.E. Analyses of
chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacum tissues with the aid
of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method. Chem. Cent. J. 2014, 8,
66. [CrossRef]
Deshpande, S.; Matei, M.F.; Jaiswal, R.; Bassil, B.S.; Kortz, U.; Kuhnert, N. Synthesis, structure, and tandem
mass spectrometric characterization of the diastereomers of quinic acid. J. Agric. Food Chem. 2016, 64,
7298–7306. [CrossRef] [PubMed]
Clifford, M.N.; Jaganath, I.B.; Ludwigc, I.A.; Crozier, A. Chlorogenic acids and the acyl-quinic acids:
Discovery, biosynthesis, bioavailability and bioactivity. Nat. Prod. Rep. 2017, 34, 1391–1421. [CrossRef]
[PubMed]
Mhlongo, M.I.; Piater, L.A.; Steenkamp, P.A.; Madala, N.E.; Dubery, I.A. Metabolomic fingerprinting of
primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid. Biotechnol.
Lett. 2015, 37, 205–209. [CrossRef]
Reddy, A.R.; Chaitanya, K.V.; Vivekanandan, M. Drought-induced responses of photosynthesis and
antioxidant metabolism in higher plants. J. Plant Physiol. 2004, 161, 1189–1202. [CrossRef]
Leone, A.; Fiorillo, G.; Criscuoli, F.; Ravasenghi, S.; Santagostini, L.; Fico, G.; Spadafranca, A.; Battezzati, A.;
Schiraldi, A.; Pozzi, F.; et al. Nutritional Characterization and Phenolic Profiling of Moringa oleifera Leaves
Grown in Chad, Sahrawi Refugee Camps, and Haiti. Int. J. Mol. Sci. 2015, 16, 18923–18937. [CrossRef]
[PubMed]
Tshabalala, T.; Ndhlala, A.R.; Ncube, B.; Abdelgadir, H.A.; Van Staden, J. Potential substitution of the root
with the leaf in the use of Moringa oleifera for antimicrobial, antidiabetic and antioxidant properties. S. Afr.
J. Bot. 2019. (In press) [CrossRef]
Plants 2019, 8, 510
53.
54.
55.
56.
57.
58.
59.
60.
61.
62.
63.
64.
65.
66.
67.
68.
69.
70.
71.
72.
73.
18 of 23
Sreelatha, S.; Padma, P.R. Antioxidant activity and total phenolic content of Moringa oleifera leaves in two
stages of maturity. Plant Foods Hum. Nutr. 2009, 64, 303–311. [CrossRef] [PubMed]
Abdulkadir, A.R.; Jahan, M.S.; Zawawi, D.D. Effect of chlorophyll content and maturity on total phenolic,
total flavonoid contents and antioxidant activity of Moringa oleifera leaf (Miracle tree). J. Chem. Pharm. Res.
2015, 75, 1147–1152.
Ndhlala, A.; Mulaudzi, R.; Ncube, B.; Abdelgadir, H.; du Plooy, C.; Van Staden, J. Antioxidant, Antimicrobial
and Phytochemical Variations in Thirteen Moringa oleifera Lam. Cultivars. Molecules 2014, 19, 10480–10494.
[CrossRef]
Sangkitikomol, W.; Rocejanasaroj, A.; Tencomnao, T. Effect of Moringa oleifera on advanced glycation
end-product formation and lipid metabolism gene expression in HepG2 cells. Genet. Mol. Res. 2014, 13,
723–735. [CrossRef]
Valentão, P.; Fernandes, E.; Carvalho, F.; Andrade, P.B.; Seabra, R.M.; Bastos, M.L. Studies on the antioxidant
activity of Lippia citriodora infusion: Scavenging effect on superoxide radical, hydroxyl radical and
hypochlorous acid. Biol. Pharm. Bull. 2002, 25, 1324–1327. [CrossRef]
Mahmood, K.T.; Mugal, T.; Haq, I.U. Moringa oleifera: A natural gift-A review. J. Pharm. Sci. Res. 2010, 2,
775–781.
Ferreira, P.M.P.; Farias, D.F.; Oliveira, J.T.D.A.; Carvalho, A.D.F.U. Moringa oleifera: Bioactive compounds and
nutritional potential. Rev. Nutr. 2008, 21, 431–437. [CrossRef]
Saini, R.K.; Manoj, P.; Shetty, N.P.; Srinivasan, K.; Giridhar, P. Relative bioavailability of folate from the
traditional food plant Moringa oleifera L. as evaluated in a rat model. J. Food Sci. Technol. 2016, 53, 511–520.
[CrossRef]
Joshi, P.; Mehta, D. Effect of dehydration on the nutritive value of drumstick leaves. J. Metabol. Syst. Biol.
2010, 1, 5–9.
Kidmose, U.; Yang, R.Y.; Thilsted, S.H.; Christensen, L.P.; Brandt, K. Content of carotenoids in commonly
consumed Asian vegetables and stability and extractability during frying. J. Food Compos. Anal. 2006, 19,
562–571. [CrossRef]
Binstock, R.H. The war on anti-aging medicine. Gerontologist 2003, 43, 4–14. [CrossRef]
Pong, K. Oxidative stress in neurodegenerative diseases: Therapeutic implications for superoxide dismutase
mimetics. Expert Opin. Biol. Ther. 2003, 3, 127–139. [CrossRef]
Ramachandran, C.; Peter, K.V.; Gopalakrishnan, P.K. Drumstick (Moringa oleifera): A multipurpose Indian
vegetable. Econ. Bot. 1980, 34, 276–283. [CrossRef]
Reed, J.D.; Soller, H.; Woodward, A. Fodder tree and straw diets for sheep: Intake, growth, digestibility and
the effects of phenolics on nitrogen utilization. Anim. Feed Sci. Technol. 1990, 30, 39–50. [CrossRef]
Bhatta, R.; Saravanan, M.; Baruah, L.; Sampath, K.T. Nutrient content, in vitro ruminal fermentation
characteristics and methane reduction potential of tropical tannin-containing leaves. J. Sci. Food Agric. 2012,
92, 2929–2935. [CrossRef]
Teixeira, E.M.B.; Carvalho, M.R.B.; Neves, V.A.; Silva, M.A.; Arantes-Pereira, L. Chemical characteristics and
fractionation of proteins from Moringa oleifera Lam. leaves. Food Chem. 2014, 147, 51–54. [CrossRef]
Moyo, B.; Masika, P.J.; Hugo, A.; Muchenje, V. Nutritional characterization of Moringa (Moringa oleifera Lam.)
leaves. Afr. J. Biotechnol. 2011, 10, 12925–12933.
Hagerman, A.E.; Riedl, K.M.; Jones, G.A.; Sovik, K.N.; Ritchard, N.T.; Hartzfeld, P.W.; Riechel, T.L. High
molecular weight plant polyphenolics (tannins) as biological antioxidants. J. Agric. Food Chem. 1998, 46,
1887–1892. [CrossRef]
Tshabalala, T.; Sikosana, J.L.N.; Chivandi, E. Nutrient intake, digestibility and nitrogen retention in indigenous
goats fed on Acacia nilotica fruits treated for condensed tannins. S. Afr. J. Anim. Sci. 2013, 43, 457–463.
[CrossRef]
Lu, W.; Wang, J.; Zhang, H.J.; Wu, S.G.; Qi, G.H. Evaluation of Moringa oleifera leaf in laying hens: Effects on
laying performance, egg quality, plasma biochemistry and organ histopathological indices. Ital. J. Anim. Sci.
2016, 15, 658–665. [CrossRef]
Tesfaye, E.B.; Animut, G.M.; Urge, M.L.; Dessie, T.A. Cassava root chips and Moringa oleifera leaf meal as
alternative feed ingredients in the layer ration1. J. Appl. Poult. Res. 2014, 23, 614–624. [CrossRef]
Plants 2019, 8, 510
74.
75.
76.
77.
78.
79.
80.
81.
82.
83.
84.
85.
86.
87.
88.
89.
90.
91.
92.
93.
94.
19 of 23
Singh, A.K.; Rana, H.K.; Tshabalala, T.; Kumar, R.; Ndhlala, A.R.; Pandey, A.K. Phytochemical, nutraceutical
and pharmacological attributes of a functional crop Moringa oleifera Lam: An overview. S. Afr. J. Bot. 2019.
[CrossRef]
Animashaun, J.O.; Williams, F.; Toye, A. Towards validating moringa′ s nutraceutical benefits: An examination
of consumers′ perspectives vis-a-vis health benefits efficacy and willingness to pay. J. Agris On-Line Pap.
Econ. Inform. 2013, 5, 11.
Mabapa, M.P.; Ayisi, K.K.; Mariga, I.K.; Mohlabi, R.C.; Chuene, R.S. Production and Utilization of Moringa
by Farmers in Limpopo Province, South Africa. Agric. Res. 2017, 12, 160–171. [CrossRef]
Saini, R.K.; Sivanesan, I.; Keum, Y.-S. Phytochemicals of Moringa oleifera: A review of their nutritional,
therapeutic and industrial significance. 3 Biotech 2016, 6, 203. [CrossRef]
Mohammed, A.; Lai, O.; Muhammad, S.; Long, K.; Ghazali, H. Moringa oleifera, potentially a new source of
oleic acid-type oil for Malaysia. Investig. Innov. 2003, 3, 137–140.
Frigolet, M.E.; Gutiérrez-Aguilar, R. The Role of the Novel Lipokine Palmitoleic Acid in Health and Disease.
Adv. Nutr. 2017, 8, 173S–181S. [CrossRef]
Jongrungruangchok, S.; Bunrathep, S.; Songsak, T. Nutrients and minerals content of eleven different samples
of Moringa oleifera cultivated in Thailand. J. Health Res. 2010, 24, 123–127.
Ntila, S.; Ndhlala, A.R.; Kolanisi, U.; Abdelgadir, H.; Siwela, M. Acceptability of a moringa-added
complementary soft porridge to caregivers in Hammanskraal, Gauteng province and Lebowakgomo,
Limpopo province, South Africa. S. Afr. J. Clin. Nutr. 2019, 32, 51–57. [CrossRef]
Abd El-Hack, M.; Alagawany, M.; Elrys, A.; Desoky, E.S.; Tolba, H.; Elnahal, A.; Elnesr, S.; Swelum, A. Effect
of Forage Moringa oleifera L.(moringa) on Animal Health and Nutrition and Its Beneficial Applications in
Soil, Plants and Water Purification. Agriculture 2018, 8, 145. [CrossRef]
Kakengi, A.; Shem, M.; Sarwatt, S.; Fujihara, T. Can Moringa oleifera be used as a protein supplement for
ruminants? Asian-australas. J. Anim. Sci. 2005, 18, 42–47. [CrossRef]
Rubanza, C.; Shem, M.; Otsyina, E.; Bakengesa, S.; Ichinohe, T.; Fujihara, T. Polyphenolics and tanninseffect
on in vitro digestibility of selected Acacia species leaves. Anim. Feed Sci. Technol. 2005, 119, 129–142.
[CrossRef]
Anwar, F.; Rashid, U. Physico-chemical characteristics of Moringa oleifera seeds and seed oil from a wild
provenance of Pakistan. Pak. J. Bot. 2007, 39, 1443–1453.
Siddhuraju, P.; Becker, K. Antioxidant properties of various solvent extracts of total phenolic constituents
from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J. Agric. Food Chem.
2003, 51, 2144–2155. [CrossRef] [PubMed]
Verma, A.R.; Vijayakumar, M.; Mathela, C.S.; Rao, C.V. In vitro and in vivo antioxidant properties of different
fractions of Moringa oleifera leaves. Food Chem. Toxicol. 2009, 47, 2196–2201. [CrossRef] [PubMed]
Lalas, S.; Tsaknis, J. Extraction and identification of natural antioxidant from the seeds of the Moringa oleifera
tree variety of Malawi. J. Am. Oil Chem. Soc. 2002, 799, 677–683. [CrossRef]
Fakurazi, S.; Sharifudin, S.A.; Arulselvan, P. Moringa oleifera hydroethanolic extracts effectively alleviate
acetaminophen-induced hepatotoxicity in experimental rats through their antioxidant nature. Molecules
2012, 17, 8334–8350. [CrossRef]
Singh, B.N.; Singh, B.; Singh, R.; Prakash, D.; Dhakarey, R.; Upadhyay, G.; Singh, H. Oxidative DNA damage
protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera. Food Chem. Toxicol.
2009, 47, 1109–1116. [CrossRef]
Moyo, B.; Oyedemi, S.; Masika, P.; Muchenje, V. Polyphenolic content and antioxidant properties of
Moringa oleifera leaf extracts and enzymatic activity of liver from goats supplemented with Moringa oleifera
leaves/sunflower seed cake. Meat Sci. 2012, 91, 441–447. [CrossRef] [PubMed]
Jayawardana, B.C.; Liyanage, R.; Lalantha, N.; Iddamalgoda, S.; Weththasinghe, P. Antioxidant and
antimicrobial activity of drumstick (Moringa oleifera) leaves in herbal chicken sausages. LWT-Food Sci.
Technol. 2015, 64, 1204–1208. [CrossRef]
Hazra, S.; Biswas, S.; Bhattacharyya, D.; Das, S.K.; Khan, A. Quality of cooked ground buffalo meat treated
with the crude extracts of Moringa oleifera (Lam.) leaves. J. Food Sci. Technol. 2012, 49, 240–245. [CrossRef]
[PubMed]
Das, A.K.; Rajkumar, V.; Verma, A.K.; Swarup, D. Moringa oleifera leaves extract: A natural antioxidant for
retarding lipid oxidation in cooked goat meat patties. Int. J. Food Sci. Technol. 2012, 47, 585–591. [CrossRef]
Plants 2019, 8, 510
95.
96.
97.
98.
99.
100.
101.
102.
103.
104.
105.
106.
107.
108.
109.
110.
111.
112.
113.
114.
115.
20 of 23
Qwele, K.; Hugo, A.; Oyedemi, S.; Moyo, B.; Masika, P.; Muchenje, V. Chemical composition, fatty acid
content and antioxidant potential of meat from goats supplemented with Moringa (Moringa oleifera) leaves,
sunflower cake and grass hay. Meat Sci. 2013, 93, 455–462. [CrossRef] [PubMed]
Feldmann, M.; Brennan, F.M.; Maini, R.N. Role of cytokines in rheumatoid arthritis. Annu. Rev. Immunol.
1996, 14, 397–440. [CrossRef]
Graves, D.T.; Liu, R.; Oates, T.W. Diabetes enhanced inflammation and apoptosis–impact on periodontal
pathosis. Periodontology 2000 2007, 45, 128–137. [CrossRef]
Araújo, L.C.C.; Aguiar, J.S.; Napoleão, T.H.; Mota, F.V.B.; Barros, A.L.S.; Moura, M.C.; Coriolano, M.C.;
Coelho, L.C.B.B.; Silva, T.G.; Paiva, P.M.G. Evaluation of cytotoxic and anti-inflammatory activities of extracts
and lectins from Moringa oleifera seeds. PLoS ONE 2013, 8, e81973. [CrossRef]
Cheenpracha, S.; Park, E.-J.; Yoshida, W.Y.; Barit, C.; Wall, M.; Pezzuto, J.M.; Chang, L.C. Potential
anti-inflammatory phenolic glycosides from the medicinal plant Moringa oleifera fruits. Bioorg. Med. Chem.
2010, 18, 6598–6602. [CrossRef]
Muangnoi, C.; Chingsuwanrote, P.; Praengamthanachoti, P.; Svasti, S.; Tuntipopipat, S. Moringa oleifera pod
inhibits inflammatory mediator production by lipopolysaccharide-stimulated raw 264.7 murine macrophage
cell lines. Inflammation 2012, 35, 445–455. [CrossRef]
Sulaiman, M.R.; Zakaria, Z.; Bujarimin, A.; Somchit, M.; Israf, D.; Moin, S. Evaluation of Moringa oleifera
aqueous extract for antinociceptive and anti-inflammatory activities in animal models. Pharm. Biol. 2008, 46,
838–845. [CrossRef]
Udupa, S.; Udupa, A.; Kulkarni, D. Studies on the anti-inflammatory and wound healing properties of
Moringa oleifera and Aegle marmelos. Fitoterapia 1994, 65, 119–123.
Vasanth, K.; Minakshi, G.; Ilango, K.; Kumar, R.M.; Agrawal, A.; Dubey, G. Moringa oleifera attenuates
the release of pro-inflammatory cytokines in lipopolysaccharide stimulated human monocytic cell line.
Ind. Crops Prod. 2015, 77, 44–50. [CrossRef]
Chuang, H.Y.; Lee, E.; Liu, Y.T.; Lee, D.; Ideker, T. Network based classification of breast cancer metastasis.
Mol. Syst. Biol. 2007, 3, 140. [CrossRef] [PubMed]
Faizi, M.S.H.; Hussain, S. Dichlorido (n, n-diethyl-4-{[(quinolin-2-yl) methylidene] amino-κ2n, n′ } aniline)
mercury (ii). Acta Cryst. 2014, 70, m197. [CrossRef] [PubMed]
Bhandari, P.; Patel, N.K.; Bhutani, K.K. Synthesis of new heterocyclic lupeol derivatives as nitric oxide and
pro-inflammatory cytokine inhibitors. Bioorg. Med. Chem. Lett. 2014, 24, 3596–3599. [CrossRef] [PubMed]
Pandith, A.A.; Shah, Z.A.; Siddiqi, M.A. Oncogenic Role of Fibroblast Growth Factor Receptor 3 in Tumorigenesis
of Urinary Bladder Cancer; Elsevier: Amsterdam, The Netherlands, 2013.
Valerio, M.; Awad, A.B. B-sitosterol down-regulates some pro-inflammatory signal transduction pathways by
increasing the activity of tyrosine phosphatase SHP-1 in J774a.1 murine macrophages. Int. Immunopharmacol.
2011, 11, 1012–1017. [CrossRef]
Cavallari, I.; Cannon, C.P.; Braunwald, E.; Goodrich, E.L.; Im, K.; Lukas, M.A.; O’donoghue, M.L. Metabolic
syndrome and the risk of adverse cardiovascular events after an acute coronary syndrome. Eur. J. Prev.
Cardiol. 2018, 25, 830–838. [CrossRef]
Grundy, S.M. Metabolic syndrome update. Trends Cardiovasc. Med. 2016, 26, 364–373. [CrossRef]
O′ neill, S.; O′ driscoll, L. Metabolic syndrome: A closer look at the growing epidemic and its associated
pathologies. Obes Res. 2015, 16, 1–12. [CrossRef]
López, M.; Ríos-Silva, M.; Huerta, M.; Cárdenas, Y.; Bricio-Barrios, J.A.; Díaz-Reval, M.I.; Urzúa, Z.;
Huerta-Trujillo, M.; López-Quezada, K.; Trujillo, X. Effects of Moringa oleifera leaf powder on metabolic
syndrome induced in male Wistar rats: A preliminary study. J. Int. Med. Res. 2018, 46, 3327–3336. [CrossRef]
[PubMed]
Villarruel-López, A.; López-de la Mora, D.; Vázquez-Paulino, O.; Puebla-Mora, A.; Torres-Vitela, M.R.;
Guerrero-Quiroz, L.; Nuño, K. Effect of Moringa oleifera consumption on diabetic rats. BMC Complement.
Altern. Med. 2018, 18, 127. [CrossRef] [PubMed]
Divi, S.; Bellamkonda, R.; Dasireddy, S.K. Evaluation of antidiabetic and antihyperlipedemic potential of
aqueous extract of Moringa oleifera in fructose fed insulin resistant and STZ induced diabetic wistar rats:
A comparative study. Asian J. Pharm. Clin. Res. 2012, 5, 67–72.
Sugunabai, J.; Jayaraj, M.; Karpagam, T.; Varalakshmi, B. Antidiabetic efficiency of Moringa oleifera and
Solanum nigrum. Int. J. Pharm. Pharm. Sci. 2014, 6, 40–42.
Plants 2019, 8, 510
21 of 23
116. Jaiswal, D.; Rai, P.K.; Kumar, A.; Mehta, S.; Watal, G. Effect of Moringa oleifera Lam.leaves aqueous extract
therapy on hyperglycemic rats. J. Ethnopharmacol. 2009, 123, 392–396. [CrossRef] [PubMed]
117. Park, A. Park′ s Textbook of Social and Preventive Medicine; Banarasidas Bhanot Publishers: Jabalpur, India, 2007.
118. WHO. World Health Organisation, Study Group on Diabetes Mellitus; WHO: Geneva, Switzerland, 1994;
pp. 78–79.
119. Francis, J.A.; Jayaprakasam, B.; Olson, L.K.; Nair, M. Insulin secretagogues from Moringa oleifera with
cyclooxygenase enzyme and lipid peroxidation inhibiting activities. Helv. Chim. Acta 2004, 87, 317–326.
[CrossRef]
120. Yassa, H.D.; Tohamy, A.F. Extract of Moringa oleifera leaves ameliorates streptozotocin-induced diabetes
mellitus in adult rats. Acta Histochem. 2014, 116, 844–854. [CrossRef]
121. Adeeyo, A.; Adefule, A.; Ofusori, D.; Aderinola, A.; Caxton-Martins, E. Antihyperglycemic effects of aqueous
leaf extracts of mistletoe and Moringa oleifera in streptozotocin-induced diabetes wistar rats. Diabetol. Croat.
2013, 42, 81–88.
122. Rudge, M.V.; Damasceno, D.C.; Volpato, G.T.; Almeida, F.C.; Calderon, I.M.; Lemonica, I.P. Effect of
Ginkgo biloba on the reproductive outcome and oxidative stress biomarkers of streptozotocin-induced diabetic
rats. Braz. J. Med. Biol. Res. 2007, 40, 1095–1099. [CrossRef]
123. Ghiridhari, V.V.A.; Malhati, D.; Geetha, K. Anti-diabetic properties of drumstick (Moringa oleifera) leaf tablets.
Int. J. Health Nutr. 2011, 2, 1–5.
124. Gupta, R.; Sharma, A.K.; Dobhal, M.; Sharma, M.; Gupta, R. Antidiabetic and antioxidant potential of
β-sitosterol in streptozotocin induced experimental hyperglycemia. J. Diabetes 2011, 3, 29–37. [CrossRef]
[PubMed]
125. Bernal-Mizrachi, E.; Wen, W.; Stahlhut, S.; Welling, C.M.; Permutt, M.A. Islet β cell expression of constitutively
active akt1/pkbα induces striking hypertrophy, hyperplasia, and hyperinsulinemia. J. Clin. Investig. 2001,
108, 1631–1638. [CrossRef] [PubMed]
126. Ross, M.H.; Pawlina, W. Histology: A Text and Atlas, with Correlated Cell and Molecular Biology; Lippincott
Williams and Wilkins: Philadelphia, PA, USA, 2001; pp. 647–664.
127. Jaiswal, D.; Rai, P.; Mehta, S.; Chatterji, S.; Shukla, S.; Rai, D.; Sharma, G.; Sharma, B.; Watal, G. Role of
Moringa oleifera in regulation of diabetes-induced oxidative stress. Asian Pac. J. Trop. Med. 2013, 6, 426–432.
[CrossRef]
128. Kumari, D.J. Hypoglycaemic effect of Moringa oleifera and Azadirachta indica in type 2 diabetes mellitus.
Bioscan 2010, 5, 211–214.
129. Ndong, M.; Uehara, M.; Katsumata, S.; Suzuki, K. Effects of oral administration of Moringa oleifera Lam on
glucose tolerance in goto-kakizaki and wistar rats. J. Clin. Biochem. Nutr. 2007, 40, 229–233. [CrossRef]
130. Khan, H.A.; Alhomida, A.S. A review of the logistic role of l-carnitine in the management of radiation toxicity
and radiotherapy side effects. J. Appl. Toxicol. 2011, 31, 707–713. [CrossRef]
131. Guevara, A.; Vargas, C.; Sakurai, H.; Fujiwara, Y.; Hashimoto, K.; Maoka, T.; Kozuka, M.; Ito, Y.; Tokuda, H.;
Nishino, H. An antitumor promoter from Moringa oleifera Lam. Mutat Res. Genet. Toxicol Environ. Mutagen.
1999, 440, 181–188. [CrossRef]
132. Murakami, A.; Kitazono, Y.; Jiwajinda, S.; Koshimizu, K.; Ohigashi, H. Niaziminin, a thiocarbamate from the
leaves of Moringa oleifera, holds a strict structural requirement for inhibition of tumor-promoter-induced
epstein-barr virus activation. Planta Med. 1998, 64, 319–323. [CrossRef]
133. Parvathy, M.; Umamaheshwari, A. Cytotoxic effect of Moringa oleifera leaf extracts on human multiple
myeloma cell lines. Trends Med. Res. 2007, 2, 44–50.
134. Jung, I.L. Soluble Extract from Moringa oleifera Leaves with a New Anticancer Activity. PLoS ONE 2014, 9,
e95492. [CrossRef]
135. Jung, I.L.; Lee, J.H.; Kang, S.C. A potential oral anticancer drug candidate, Moringa oleifera leaf extract,
induces the apoptosis of human hepatocellular carcinoma cells. Oncol. Lett. 2015, 10, 1597–1604. [CrossRef]
[PubMed]
136. Muyibi, S.A.; Evison, L.M. Optimizing physical parameters affecting coagulation of Turbid water with
Moringa oleifera seeds. Water Research 1995, 29, 2689–2695. [CrossRef]
137. Vasanth, K.; Ilango, K.; MohanKumar, R.; Agrawal, A.; Dubey, G.P. Anticancer activity of Moringa oleifera
mediated silver nanoparticles on human cervical carcinoma cells by apoptosis induction. Colloids Surf. B
2014, 117, 354–359. [CrossRef] [PubMed]
Plants 2019, 8, 510
22 of 23
138. Tahiliani, P.; Kar, A. Role of Moringa oleifera leaf extract in the regulation of thyroid hormone status in adult
male and female rats. Pharmacol. Res. 2000, 41, 319–323. [CrossRef] [PubMed]
139. Das, N.; Sikder, K.; Ghosh, S.; Fromenty, B.; Dey, S. Moringa oleifera Lam. leaf extract prevents early liver
injury and restores antioxidant status in mice fed with high-fat diet. Indian J. Exp. Biol. 2012, 50, 404–412.
[PubMed]
140. Kelly, G. Peripheral metabolism of thyroid hormones: A review. Altern. Med. Rev. 2000, 5, 306.
141. Sileshi, T.; Makonnen, E.; Debella, A.; Tesfaye, B. Antihyperglycemic and subchronic toxicity study of
Moringa stenopetala leaves in mice. J. Coast. Life Med. 2014, 2, 214–221.
142. Gholap, S.; Kar, A. Hypoglycaemic effects of some plant extracts are possibly mediated through inhibition in
corticosteroid concentration. Pharmazie 2004, 59, 876–878.
143. Barichella, M.; Pezzoli, G.; Faierman, S.A.; Raspini, B.; Rimoldi, M.; Cassani, E.; Bertoli, S.; Battezzati, A.;
Leone, A.; Iorio, L. Nutritional characterisation of Zambian Moringa oleifera: Acceptability and safety of
short-term daily supplementation in a group of malnourished girls. Int. J. Food Sci. Nutr. 2019, 70, 107–115.
[CrossRef] [PubMed]
144. Stohs, S.J.; Hartman, M.J. Review of the safety and efficacy of Moringa oleifera. Phytother. Res. 2015, 29,
796–804. [CrossRef]
145. Annongu, A.; Karim, O.; Toye, A.; Sola-Ojo, F.; Kayode, R.; Badmos, A.; Alli, O.; Adeyemi, K. Geo-Assessment
of chemical composition and nutritional Evaluation of Moringa oleifera seeds in nutrition of Broilers.
J. Agric. Sci. 2014, 6, 119. [CrossRef]
146. Maizuwo, A.I.; Hassan, A.S.; Momoh, H.; Muhammad, J.A. Phytochemical Constituents, Biological Activities,
Therapeutic Potentials and Nutritional Values of Moringa oleifera (Zogale): A Review. J. Drug Des. Med. 2017,
3, 60.
147. Dutta, A.K. Moringa oleifera: A Review on its importance and medicinal applications in recent age. World J.
Pharm. Pharm. Sci. 2017, 6, 1829–1843.
148. Adedapo, A.; Mogbojuri, O.; Emikpe, B. Safety evaluations of the aqueous extract of the leaves of Moringa
oleifera in rats. J. Med. Plants Res. 2009, 3, 586–591.
149. Pritchard, M.; Mkandawire, T.; Edmondson, A.; O′ neill, J.G.; Kululanga, G. Potential of using plant extracts
for purification of shallow well water in Malawi. Phys. Chem. Earth Parts A B C 2009, 34, 799–805. [CrossRef]
150. Amagloh, F.K.; Benang, A. Effectiveness of Moringa oleifera seed as coagulant for water purification. Afr. J.
Agric. Res. 2009, 4, 119–123.
151. Sotheeswaran, S.; Nand, V.; Matakite, M.; Kanayathu, K. Moringa oleifera and other local seeds in water
purification in developing countries. Res. J. Chem. Environ. 2011, 15, 135–138.
152. Muyibi, S.A.; Megat, J.M.M.N.; Lam, H.L.; Tan, K.L. Effects of oil extraction from Moringa oleifera seeds on
coagulation of turbid water. Int. J. Environ. Stud. 2002, 59, 243–254. [CrossRef]
153. Ndabigengesere, A.; Narasiah, K.S.; Talbot, B.G. Active agent and mechanism of coagulation of turbid waters
using Moringa oleifera. Water Res 1995, 2, 703–710. [CrossRef]
154. Pritchard, M.; Craven, T.; Mkandawire, T.; Edmondson, A.S.; O′ neill, J.G. A study of the parameters affecting
the effectiveness of Moringa oleifera in drinking water purification. Phys. Chem. Earth Parts A B C 2010, 35,
791–797. [CrossRef]
155. Mangale, S.M.; Chonde, S.G.; Jadhav, A.S.; Raut, P.D. Study of Moringa oleifera (drumstick) seed as natural
absorbent and antimicrobial agent for river water treatment. J. Nat. Prod. Plant Resour. 2012, 2, 89–100.
156. Bakare, B.F. An Investigation of Moringa oleifera Seed Extract as a Natural Coagulant in Water Treatment. In
Proceedings of the World Congress on Engineering and Computer Science, San Francisco, CA, USA, 19–21
October 2016.
157. Schwarz, D. Water clarification using Moringa olifera. Technol. Inform. 2000, 1, 17–20. [CrossRef]
158. Scholes, R.J.; Archer, S.R. Tree-grass interactions in savannas. Annu. Rev. Ecol. Evol. Syst. 1997, 28, 517–544.
[CrossRef]
159. Tshabalala, T.; Ncube, B.; Moyo, H.P.; Abdel-Rahman, E.M.; Mutanga, O.; Ndhlala, A.R. Predicting the spatial
suitability distribution of Moringa oleifera cultivation using analytical hierarchical process modelling. S. Afr.
J. Bot. 2019. (In press) [CrossRef]
160. Zaku, S.G.; Emmanuel, S.; Tukur, A.A.; Kabir, A. Moringa oleifera: An underutilized tree in Nigeria with
amazing versatility: A review. Afr. J. Food Sci. 2015, 9, 456–461.
Plants 2019, 8, 510
23 of 23
161. Bridgemohan, P.; Bridgemohan, R.S.H. Invasive weed risk assesment of three potential bioenerygy fuel
species. Int. J. Biodivers. Conserv. 2014, 6, 790–796.
162. Nahar, S.; Faisal, F.M.; Iqbal, J.; Rahman, M.; Yusuf, A. Antiobesity activity of Moringa oleifera leaves against
high fat diet-induced obesity in rats. Int. J. Basic Clin. Pharmacol. 2016, 5, 1263–1268. [CrossRef]
163. Navie, S.; Csurhes, S. Invasive Plant Risk Assessment: Moringa Oleifera; Department of Agriculture and Fisheries
Biosecurity Queensland: Biosecurity Queensland: Brisbane, Queensland, Australia, 2016.
164. CABI. Invasive Species Compendium. Available online: https://www.cabi.org/isc/datasheet/34868 (accessed
on 22 June 2018).
165. Fuglie, L.J. New Uses of Moringa Studied in Nica Ragua: ECHO′ s Technical Network Site-Networking Global
Hunger Solutions; ECHO: North Fort Myers, FL, USA, 2010.
166. Mehboob, W.; Rehman, H.; Basra, S.M.A.; Afzal, I. Role of seed priming in improving performance of spring
maize. In Proceedings of the International Seminar on Crop Management: Issues and Options, University of
Agriculture, Faisalabad, Pakistan, 1–2 June 2011; p. 55.
167. Soliman, M.H.; Ahlam, H.H.; Hamdah, A.G.; Shroug, S. Allelopathic Effect of Moringa oleifera Leaves Extract
on Seed Germination and Early Seedling Growth of Faba Bean (Vicia faba L.). Int. J. Agric. Technol. 2017, 13,
105–117.
168. Iqbal, M.A. Role of moringa, brassica and sorghum water extracts in increasing crops growth and yield:
A Review. Am. Eurasian J. Agric. Environ. Sci. 2014, 14, 1150–1158.
169. Nouman, W.; Siddiqui, M.T.; Basra, S.M.A. Moringa oleifera leaf extract: An innovative priming tool for
rangeland grasses. Turk. J. Agric. For. 2012, 35, 65–75.
170. Phiri, C. Influence of Moringa oleifera leaf extracts on germination and early seedling development of major
cereals. Agric. Biol. J. N Am. 2010, 1, 774–777. [CrossRef]
171. Hossain, M.M.; Miah, G.; Ahamed, T.; Sarmin, N.S. Study on allelopathic effect of Moringa oleifera on the
growth and productivity of mungbean. Int. J. Agric. Crop. Sci. 2012, 4, 1122–1128.
172. Tahir, N.A.; Qader, K.O.; Azeez, H.A.; Rashid, J.S. Inhibitory allelopathic effects of Moringa oleifera Lamk
plant extracts on wheat and Sinapis arvensis L. Allelopath. J. 2018, 44, 35–48. [CrossRef]
173. Hussain, M.; Farooq, M.; Basra, S.M.; Lee, D.J. Application of Moringa allelopathy in crop sciences.
In Allelopathy; Springer: Berlin/Heidelberg, Germany, 2013; pp. 469–483.
174. Iqbal, M.A. Managing sunflower (Helianthus annuus L.) nutrition with foliar application of moringa
(Moringa oleifera Lam.) leaf extract. Am. Eurasian J. Agric. Environ. Sci. 2014, 14, 1339–1345.
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