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Optimization of extraction process and antioxidant activities of saponins from Camellia fascicularis leaves

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Abstract

Saponins from Camellia fascicularis leaves, which ultrasonic-assisted extraction process parameters were optimized with Box–Behnken design and antioxidant activities were assessed, were reported in this study. The highest yield of crude saponins was up to 3.68% when the optimal extraction conditions were ratio of solvent to solid of 52.1 mL/g, methanol concentration of 71.1%, and ultrasonic temperature of 50.3 °C. The crude saponins from C. fascicularis leaves were purified using HP-20 macroporous resin with methanol and three fractions (SCF-1, SCF-2 and SCF-3) were obtained. Subsequently, the in vitro scavenging activity assay showed that the fractions proved significant antioxidant capacities as dose-dependent manner on 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). SCF-1 possessed significantly higher antioxidant activity than that SCF-2 and SCF-3. Overall, ultrasonic-assisted extraction could be effectively used to extract C. fascicularis leaves saponins, which possessed potential antioxidant capabilities and is expected to be developed as food materials.

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References

  1. T.L. Min, B. Bartholomew, Theaceae, Camellia, in Flora of China, vol. 12, ed. by Z.Y. Wu, P.H. Raven, D.Y. Hong (Missouri Botanical Garden Press, St. Louis, 2007), pp. 367–372

    Google Scholar 

  2. Y. Xie, S. Wang, Conservation status of Chinese species: (1) overview. Integr. Zool. 2(1), 26–35 (2007)

    Article  Google Scholar 

  3. J.N. Lin, H.Y. Lin, N.S. Yang, Y.H. Li, M.R. Lee, C.H. Chuang, C.T. Ho, S.C. Kuo, T.D. Way, Chemical constituents and anticancer activity of yellow camellias against MDA-MB-231 human breast cancer cells. J. Agr. Food Chem. 61(40), 9638–9644 (2013)

    CAS  Google Scholar 

  4. H. Oku, Y. Ogawa, E. Iwaoka, Y. Yamaguchi, S. Kagota, S. Kazumasa, M. Kunitomo, K. Ishiguro, Preventive effects of the extract of kinka-cha, a folk tea, on a rat model of metabolic syndrome. J. Nat. Med. 65(3–4), 610–616 (2011)

    Article  Google Scholar 

  5. X. Peng, D.Y. Yu, B.M. Feng, Y.Q. Wang, L.Y. Shi, A new acylated flavonoid glycoside from the flowers of camellianitidissima and its effect on the induction of apoptosis in human lymphoma U937 cells. J. Asian Nat. Prod. Res. 14(8), 799–804 (2012)

    Article  CAS  Google Scholar 

  6. J. Qi, R.F. Shi, J.M. Yu, Y. Li, S.T. Yuan, J.Z. Yang, J.M. Hu, A.Q. Jia, Chemical constituents from leaves of Camellianitidissima, and their potential cytotoxicity on SGC7901 cells. Chin. Herb. Med. 8(1), 80–84 (2016)

    Article  CAS  Google Scholar 

  7. L.X. Song, X.S. Wang, X.Q. Zheng, D.J. Huang, Polyphenolic antioxidant profiles of yellow camellia. Food Chem. 129(2), 351–357 (2011)

    Article  CAS  Google Scholar 

  8. J.B. Wei, X. Li, H. Song, Y.H. Liang, Y.Z. Pan, J.X. Ruan, X. Qin, Y.X. Chen, C.L. Nong, Characterization and determination of antioxidant components in the leaves of Camelliachrysantha (Hu) Tuyama based on composition–activity relationship approach. J. Food Drug Anal. 23(1), 40–48 (2015)

    Article  CAS  Google Scholar 

  9. L.H. Lei, Y.L. Zhang, L.X. Niu, L.H. Li, X.X. Zhang, Antioxidant activity and active components of Lilium lancifolium Thunb. bulbs from 15 populations. Food Sci. 36(14), 122–129 (2015)

    CAS  Google Scholar 

  10. S.Q. Liu, Z.B. Tan, P.T. Li, X.L. Gao, Y.E. Zeng, S.L. Wang, HepG2 cells biospecific extraction and HPLC-ESI-MS analysis for screening potential antiatherosclerotic active components in Bupeuriradix. J. Pharmaceut. Biomed. 121, 56–62 (2016)

    Article  CAS  Google Scholar 

  11. P.M. Pauletti, A.R. Araújo, M.C.M. Young, A.M. Giesbrecht, V.D.S. Bolzani, Nor-lignans from the leaves of Styraxferrugineus (Styracaceae) with antibacterial and antifungal activity. Phytochemistry 55(6), 597–601 (2000)

    Article  CAS  Google Scholar 

  12. M.H. Pan, C.S. Lai, S. Dushenkov, C.T. Ho, Modulation of inflammatory genes by natural dietary bioactive compounds. J. Agr. Food Chem. 57(11), 4467–4477 (2009)

    Article  CAS  Google Scholar 

  13. L. Chen, J.G. Dai, Z.L. Wang, H.Y. Zhang, Y.F. Huang, Y.N. Zhao, The antidepressant effects of ginseng total saponins in male C57BL/6N mice by enhancing hippocampal inhibitory phosphorylation of GSK-3β. Phytother. Res. 28(7), 1102–1106 (2014)

    Article  CAS  Google Scholar 

  14. X. Xiao, P. Bai, T.M. Bui Nguyen, J.G. Xiao, S.L. Liu, G. Yang, L.N. Hu, X.L. Chen, X.M. Zhang, J.S. Liu, H. Wang, The antitumoral effect of Paris Saponin I associated with the induction of apoptosis through the mitochondrial pathway. Mol. Cancer Ther. 8(5), 1179–1188 (2009)

    Article  CAS  Google Scholar 

  15. Z.L. Zhou, C.Y. Li, L.H. Chen, Y.L. Yang, W.Q. Yin, B. Liu, Biotransformation of natural saponins. Chin. J. Exp. Trad. Med. For. 5(16), 173–192 (2019)

    Google Scholar 

  16. W. Dong, Y.G. Gao, Z.M. He, Y. Zhao, L.X. Zhang, Research progress in extraction and separation technology of Ginsenosides. Sci. Tech. Food Ind. 35(17), 366–369 (2014)

    CAS  Google Scholar 

  17. X.L. Shi, Y.R. Jin, J.B. Liu, H.Y. Zhou, W. Wei, H.Q. Zhang, X.W. Li, Matrix solid phase dispersion extraction of ginsenosides in the leaves of Panaxginseng C M. Mey. Food Chem. 129(3), 1253–1257 (2011)

    Article  CAS  Google Scholar 

  18. Y.T. Wang, J.Y. You, Y. Yu, C.L. Qu, H.R. Zhang, L. Ding, H.Q. Zhang, X.W. Li, Analysis of ginsenosides in Panaxginseng in high pressure microwave-assisted extraction. Food Chem. 110(1), 161–167 (2008)

    Article  CAS  Google Scholar 

  19. W. Chen, W.P. Wang, H.S. Zhang, Q. Huang, Optimization of ultrasonic-assisted extraction of water-soluble polysaccharides from Boletusedulis mycelia using response surface methodology. Carbohydr. Polym. 87(1), 614–619 (2012)

    Article  CAS  Google Scholar 

  20. A. Ciric, B. Krajnc, D. Heath, N. Ogrinc, Response surface methodology and artificial neural network approach for the optimization of ultrasound-assisted extraction of polyphenols from garlic. Food Chem. Toxicol. 135, 110976 (2020)

    Article  CAS  Google Scholar 

  21. K. Vilkhu, R. Mawson, L. Simons, D. Bates, Applications and opportunities for ultrasound assisted extraction in the food industry: a review. Innov. Food Sci. Emerg. 9(2), 161–169 (2008)

    Article  CAS  Google Scholar 

  22. W.Y. Cui, S.X. Liu, Y.M. Zhao, T.J. Zhang, X.K. Song, D.L. Liu, N.Q. Sun, Y.T. Zheng, Optimization of extraction technology of total saponins from Patriniascabiosaefolia by response surface method and its contraction effect on isolated rat intestinal smooth muscle. Chin. Trad. Herb. Drugs 47(12), 2078–2083 (2016)

    Google Scholar 

  23. S. Ahluwalia, R. Bidlan, A.K. Shrivastav, R.K. Goswami, P. Singh, J.G. Sharma, Optimization of protein extraction from detoxified Jatropha seed cake using response surface methodology and amino acid analysis. Int. J. Environ. Sci. Te. 17(2), 1087–1100 (2020)

    Article  CAS  Google Scholar 

  24. M. Hadidi, A. Ibarz, J. Pagan, Optimisation and kinetic study of the ultrasonic-assisted extraction of total saponins from alfalfa (Medicagosativa) and its bioaccessibility using the response surface methodology. Food Chem. 309, 125786 (2020)

    Article  CAS  Google Scholar 

  25. E.S. Liu, M.X. Li, A. Abdella, M.R. Wilkins, Development of a cost-effective medium for submerged production of fungal aryl alcohol oxidase using a genetically modified Aspergillusnidulans strain. Bioresource Technol. 305, 123038 (2020)

    Article  CAS  Google Scholar 

  26. S.L. Feng, H.R. Cheng, L. Fu, C.B. Ding, L. Zhang, R.W. Yang, Y.H. Zhou, Ultrasonic-assisted extraction and antioxidant activities of polysaccharides from Camelliaoleifera leaves. Int. J. Biol. Macromol. 68, 7–12 (2014)

    Article  CAS  Google Scholar 

  27. S. Kandi, A.L. Charles, Statistical comparative study between the conventional DPPH spectrophotometric and dropping DPPH analytical method without spectrophotometer: evaluation for the advancement of antioxidant activity analysis. Food Chem. 287, 338–345 (2019)

    Article  CAS  Google Scholar 

  28. S.A. Shen, H.R. Cheng, X. Li, T. Li, M. Yuan, Y.H. Zhou, C.B. Ding, Effects of extraction method on antioxidant activities of polysaccharides from camellia seed cake. Eur. Food Res. Technol. 238(6), 1015–1021 (2014)

    Article  CAS  Google Scholar 

  29. H.Y. Chen, G.C. Yen, Antioxidant activity and free radical-scavenging capacity of extracts from guava (Psidium Guajava L.) leaves. Food Chem. 101(2), 686–694 (2007)

    Article  CAS  Google Scholar 

  30. L. Yang, Y.L. Cao, J.G. Jiang, J. Chen, L. Zhu, Response surface optimization of ultrasound-assisted flavonoids extraction from the flower of Citrus aurantium L. var. amara engl. J. Sep. Sci. 33(9), 1349–1355 (2010)

    Article  CAS  Google Scholar 

  31. M.X. Li, K. Eskridge, E.S. Liu, M. Wilkins, Enhancement of polyhydroxybutyrate (PHB) production by 10-fold from alkaline pretreatment liquor with an oxidative enzyme-mediator-surfactant system under Plackett–Burman and central composite designs. Bioresource Technol. 281, 99–106 (2019)

    Article  CAS  Google Scholar 

  32. H.V. Annegowda, R. Bhat, L.M. Tze, A.A. Karim, S.M. Mansor, The free radical scavenging and antioxidant activities of pod and seed extract of Clitoriafairchildiana (howard)- an underutilized legume. J. Food Sci. Tech. 50(3), 535–541 (2013)

    Article  CAS  Google Scholar 

  33. J.J. Guo, X. Lei, D.Y. Ren, H.Y. Yang, X.B. Yang, Study on purification and antioxidant activity of total saponins of gynostemma tea. Sci. Tech. Food Ind. 36(5), 99–102 (2015)

    CAS  Google Scholar 

  34. E.C. Ning, M. Xin, L. Wei, X.M. Qin, Study on the antioxidant activity of saponins from CamelliaChrysantha (Hu) Tuyama. Food Sci. Tech. 34(11), 197–199 (2009)

    CAS  Google Scholar 

  35. A. Raza, F. Li, X.Q. Xu, J. Tang, Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapaquadrispinosa using response surface methodology. Int. J. Biol. Macromol. 94, 335–344 (2017)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by Yunnan Agricultural Basic Research Joint Project (Grant No. 2017FG001-084). The authors gratefully acknowledge the support of Ling Fu, Dongqiong Zhu and Chenggong Hu during this research.

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Authors and Affiliations

  1. Key Laboratory for Forest Resources Conservation and Utilisation in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, 650224, China

    Xiang Hu, Jun-rong Tang, Jia Deng, Huan Kan, Ping Zhao & Yun Liu

  2. College of Horticulture and Landscape, Southwest Forestry University, Kunming, 650224, China

    Xiang Hu

  3. Forestry and Grassland Bureau of Hekou County, Hekou, 661399, China

    Gui-liang Zhang

  4. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China

    Ying-jun Zhang

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  1. Xiang Hu
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Correspondence to Ping Zhao or Yun Liu.

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Hu, X., Tang, Jr., Zhang, Gl. et al. Optimization of extraction process and antioxidant activities of saponins from Camellia fascicularis leaves. Food Measure 15, 1889–1898 (2021). https://doi.org/10.1007/s11694-020-00754-0

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  • DOI: https://doi.org/10.1007/s11694-020-00754-0

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