Abstract
Saponins from Camellia fascicularis leaves, which ultrasonic-assisted extraction process parameters were optimized with Box–Behnken design and antioxidant activities were assessed, were reported in this study. The highest yield of crude saponins was up to 3.68% when the optimal extraction conditions were ratio of solvent to solid of 52.1 mL/g, methanol concentration of 71.1%, and ultrasonic temperature of 50.3 °C. The crude saponins from C. fascicularis leaves were purified using HP-20 macroporous resin with methanol and three fractions (SCF-1, SCF-2 and SCF-3) were obtained. Subsequently, the in vitro scavenging activity assay showed that the fractions proved significant antioxidant capacities as dose-dependent manner on 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). SCF-1 possessed significantly higher antioxidant activity than that SCF-2 and SCF-3. Overall, ultrasonic-assisted extraction could be effectively used to extract C. fascicularis leaves saponins, which possessed potential antioxidant capabilities and is expected to be developed as food materials.
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Acknowledgements
This research was supported by Yunnan Agricultural Basic Research Joint Project (Grant No. 2017FG001-084). The authors gratefully acknowledge the support of Ling Fu, Dongqiong Zhu and Chenggong Hu during this research.
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Hu, X., Tang, Jr., Zhang, Gl. et al. Optimization of extraction process and antioxidant activities of saponins from Camellia fascicularis leaves. Food Measure 15, 1889–1898 (2021). https://doi.org/10.1007/s11694-020-00754-0
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DOI: https://doi.org/10.1007/s11694-020-00754-0