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Biostimulant activity of sulfated polysaccharide extract from red seaweed Halymenia dilatata on yield of Mung bean in greenhouse conditions

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Abstract

The growth-promoting activity of the sulfated polysaccharides isolated from the red alga Halymenia dilatata was evaluated on the yield of mung bean Vigna radiata (L.). The heterogeneous structure of H. dilatata polysaccharide containing α-D-mannuronic acid, (1–6)-d-linked galactons, and sulfate complex was detected by FTIR and 13C NMR. Mung bean seeds were primed with different concentrations (0–2% w/v) of sulfated polysaccharide extract and seeds treated with 1.5% polysaccharide showed maximum 90% germination. The seedlings treated with 1.5% of polysaccharide recorded the maximum shoot length (28.52 cm), root length (16.54 cm), leaf area (28.25 mm2), fresh weight (0.93 g), and dry weight (0.49 g) Significantly (P < 0.05) higher amounts of total chlorophylls, carbohydrate, and protein were detected in the 1.5% polysaccharide-treated plants compared to the control. Furthermore, the number of flowers (15), pod length (7.9 cm), pod weight (0.41 g), and number of seeds (14) per pod were also significantly increased in the 1.5% polysaccharide-treated plants. Hence, our investigation showed that the application of H. dilatata polysaccharide extract could be a potent and eco-friendly biostimulant for improving mung bean growth and yield.

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Funding

This study is funded by the University Grants Commission (UGC), New Delhi, India, under UGC- Special Assistance Programme, DRS-I.

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Authors and Affiliations

  1. Department of Biotechnology, Sona College of Arts and Science, Salem, 636 005, Tamil Nadu, India

    S. Vinoth

  2. Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    P. Gurusaravanan & P. Gowtham

  3. Department of Biotechnology, School of Life Sciences, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    M. Arun

  4. Department of Chemistry, School of Life Sciences, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    M. Saradhadevi

  5. Department of Botany (PG), Ayya Nadar Janaki Ammal College, Sivakasi, 626124, Tamil Nadu, India

    N. Senthilkumar

  6. Department of Botany, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    S. R. Sivakumar

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  1. S. Vinoth
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Contributions

Gurusaravanan and Vinoth Contributed equally to this work in methodology designing, validation, and writing the manuscript. Arun, Saradhadevi, and Senthilkumar have critically evaluated the manuscript. Gowtham has contributed in the field evaluation study.

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Correspondence to S. Vinoth.

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Vinoth, S., Gurusaravanan, P., Arun, M. et al. Biostimulant activity of sulfated polysaccharide extract from red seaweed Halymenia dilatata on yield of Mung bean in greenhouse conditions. J Appl Phycol 33, 3309–3317 (2021). https://doi.org/10.1007/s10811-021-02507-y

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  • DOI: https://doi.org/10.1007/s10811-021-02507-y

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