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Physical Exercise and Natural and Synthetic Products in Health and Disease pp 287–298Cite as

Testing the Effect of Curcumin on Proliferative Capacity of Colorectal Cancer Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2343))

Abstract

This chapter presents a protocol for studying the effects of curcumin in a colorectal cell line and a mouse model of colitis-associated colon carcinogenesis. The protocol using the CT26 cell line incorporates cell proliferation, migration, invasion, spheroid formation, cell cycle, polymerase chain reaction (PCR), and western blot analyses. For the mouse model, this involved a macroscopic and histological examination of the colon and assays for oxidative damage markers.

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Author information

Authors and Affiliations

  1. Department of Food Science and Technology, Quchan Branch, Islamic Azad University, Quchan, Iran

    Tannaz Jamialahmadi

  2. Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Tannaz Jamialahmadi

  3. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil

    Paul C. Guest

  4. Charlesworth House, Debden, Essex, UK

    Paul C. Guest

  5. Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran

    Amir R. Afshari

  6. Sabinsa Corporation, East Windsor, NJ, USA

    Muhammed Majeed

  7. Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Amirhossein Sahebkar

  8. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Amirhossein Sahebkar

  9. School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Amirhossein Sahebkar

Authors
  1. Tannaz Jamialahmadi
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  2. Paul C. Guest
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  3. Amir R. Afshari
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  4. Muhammed Majeed
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  5. Amirhossein Sahebkar
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Corresponding author

Correspondence to Amirhossein Sahebkar .

Editor information

Editors and Affiliations

  1. Charlesworth House, Debden, Essex, UK

    Paul C. Guest

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Muhammed Majeed is the founder of Sabinsa-Sami Ltd.

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Jamialahmadi, T., Guest, P.C., Afshari, A.R., Majeed, M., Sahebkar, A. (2022). Testing the Effect of Curcumin on Proliferative Capacity of Colorectal Cancer Cells. In: Guest, P.C. (eds) Physical Exercise and Natural and Synthetic Products in Health and Disease. Methods in Molecular Biology, vol 2343. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1558-4_20

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  • DOI: https://doi.org/10.1007/978-1-0716-1558-4_20

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1557-7

  • Online ISBN: 978-1-0716-1558-4

  • eBook Packages: Springer Protocols

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