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Experimental and Computational Evaluation of Extraction Procedure and Scavenging Capacity of Sweet Basil Extracts (Ocimum basilicum L.)

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Abstract

The possibility to prevent nutrition-related diseases that include scavenge of free radicals and to block chain reactions is very important and significant for human well-being. The aim of this study was to analyse different basil extracts, determine the relationship between total phenolic/flavonoid content and antioxidant activity in order to optimize its application in industry. The extraction involved different solvents (ethanol, methanol and water), extraction time (10 and 30 min and 24, 48 and 72 h), plant fragmentation level (0.3 and 2 mm) and the presence or absence of light. Antioxidant activity was investigated by applying spectrophotometric method and measuring the total phenolic and flavonoid content and DPPH radical scavenging activity. The content of total phenolics varied from 5.2 to 185.6 mg of gallic acid equivalents per gram of a dry extract and flavonoids ranged from 0.2 to 35.0 mg of quercetin per gram of a dry extract. All extracts presented a scavenging capacity and IC50 values of DPPH radical inhibition ranged from 0.04 to 12.99 μg/ml. The evaluation of experimental data for eighty basil extracts was performed by chemometric analysis showing good correlation between yield and total phenolic compounds, as well as flavonoid content and inhibition of the DPPH radical.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The work was financially supported by the Ministry of Science and Technological Development of the Republic of Serbia (Project No. 172021 and 172013) and III46009.

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Authors and Affiliations

  1. Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad, Serbia

    Branislava Teofilović, Emilia Gligorić & Aleksandar Rašković

  2. Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 3, Novi Sad, Serbia

    Nevena Grujić-Letić, Ružica Igić, Gyöngyi Vastag & Slobodan Gadžurić

Authors
  1. Branislava Teofilović
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  2. Nevena Grujić-Letić
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  3. Emilia Gligorić
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  4. Aleksandar Rašković
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  5. Ružica Igić
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  6. Gyöngyi Vastag
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  7. Slobodan Gadžurić
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Correspondence to Branislava Teofilović.

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Teofilović, B., Grujić-Letić, N., Gligorić, E. et al. Experimental and Computational Evaluation of Extraction Procedure and Scavenging Capacity of Sweet Basil Extracts (Ocimum basilicum L.). Plant Foods Hum Nutr 76, 240–247 (2021). https://doi.org/10.1007/s11130-021-00902-x

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