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High Hydrostatic Pressure-Assisted Extraction of Carotenoids from Papaya (Carica papaya L. cv. Maradol) Tissues Using Soybean and Sunflower Oil as Potential Green Solvents

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Abstract

Carotenoids are health promoting compounds which bioavailability depends on their release from the intracellular compartments. The aim of this study was to evaluate the effect of the application of different levels of pressure, time and temperature in High Hydrostatic Pressure-Assisted Extraction (HHPAE) processes, and the use of soybean and sunflower oils on the extraction yield of carotenoids present in papaya pulp and peel. . Papaya (pulp and peel) as source of carotenoids and soybean and sunflower oils as eco-friendly solvents were used. The effects of temperature, pressure and time (20–40 °C, 300–500 MPa and 2–8 min) on the carotenoid extraction yield were studied applying a central composite experimental design. Carotenoids and esters in papaya were analysed by high performance liquid chromatography (HPLC-DAD-MS/MS) and by spectrophotometric methods. The main carotenoids found in carotenoid-rich oils were (all-E)-lycopene, (13Z)-lycopene isomer, β-carotene, (all-E)-β-cryptoxanthin and xanthophyll esters as (all-E)-β-cryptoxanthin caprate, (all-E)-β-cryptoxanthin laurate and (all-E)-β-cryptoxanthin myristate. The optimal extraction condition was obtained at 400 MPa, 40.5 °C for 5 min, with the highest (all-E)-lycopene extraction yield (99.1%) from papaya pulp using soybean oil. The highest (all-E)-β-carotene (14.0%) and (all-E)-β-cryptoxanthin (19.3%) extraction yields were obtained from peel extracts at 500 MPa, 35 °C for 2 min using soybean oil and 400 MPa, 27.5 °C for 5 min using sunflower oil, respectively. The results showed that carotenoid extraction yields in papaya pulp and peel extracts were higher applying mild pressures (300–400 MPa). Low xanthophyll and xanthophyll esters extraction efficiency (0.8–3.1%) was observed due to the low polarity of the vegetable oils.

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Funding

This research was funded by the Spanish Ministry of Science and Innovation (Spain), through project number RTA2015-00044-C02-02 and the Tecnológico de Monterrey, Mexico (FunFoodEmertec). Author Sara Lara-Abia received financial support from Tecnológico de Monterrey (México) (Research Chair Funds CAT-200) and CONACYT-SEP (Research Project 101700 and Doctoral Scholarship no. 895077).

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Authors and Affiliations

  1. Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL), (CSIC-UAM), Madrid, Spain

    Sara Lara-Abia, Andrea Gomez-Maqueo & M. Pilar Cano

  2. Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Monterrey, NL, México

    Sara Lara-Abia, Andrea Gomez-Maqueo, Jorge Welti-Chanes & M. Pilar Cano

  3. Permanent address: Food Structure Team, Clinical Nutrition Research Center, Singapore Institute of Food and Biotechnology Innovation, Agency for Science, Research and Technology, Singapore, Singapore

    Andrea Gomez-Maqueo

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  1. Sara Lara-Abia
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Correspondence to M. Pilar Cano.

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Lara-Abia, S., Gomez-Maqueo, A., Welti-Chanes, J. et al. High Hydrostatic Pressure-Assisted Extraction of Carotenoids from Papaya (Carica papaya L. cv. Maradol) Tissues Using Soybean and Sunflower Oil as Potential Green Solvents. Food Eng Rev 13, 660–675 (2021). https://doi.org/10.1007/s12393-021-09289-6

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