Extraction of sunflower head pectin with superfine grinding pretreatment

Jing Tan; Xiao Hua; Jingran Liu; Mingming Wang; Yaxian Liu; Ruijin Yang; Yanping Cao

doi:10.1016/j.foodchem.2020.126631

Extraction of sunflower head pectin with superfine grinding pretreatment

Food Chem. 2020 Aug 1:320:126631. doi: 10.1016/j.foodchem.2020.126631. Epub 2020 Mar 16.

Authors

Jing Tan¹, Xiao Hua², Jingran Liu¹, Mingming Wang¹, Yaxian Liu¹, Ruijin Yang³, Yanping Cao⁴

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, 214122 Wuxi, China; School of Food Science and Technology, Jiangnan University, 214122 Wuxi, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, 214122 Wuxi, China; School of Food Science and Technology, Jiangnan University, 214122 Wuxi, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China. Electronic address: huaxiao@jiangnan.edu.cn.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, 214122 Wuxi, China; School of Food Science and Technology, Jiangnan University, 214122 Wuxi, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China.
⁴ Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China.

PMID: 32222660
DOI: 10.1016/j.foodchem.2020.126631

Abstract

Effect of superfine grinding on pectin extraction was investigated. Sunflower heads were grinded into microparticles of ~50 μm, thus pectin chains were fully exposed due to cell wall breakdown. A good pectin yield of 14.5 ± 0.36% (w/w) was subsequently achieved at mild conditions (pH 5.0, 25 °C, 2 h) associated with 0.8% (w/v) sodium citrate (SC). However, the molecule weight of pectin was greatly reduced (M_w = 7.87 ± 0.21 kDa) due to the action of endo-pectinases. With heating (pH 5.0, 85 °C for 20 min, 25 °C for 1.5 h) the endo-pectinases were effectively inhibited, thus M_w was increased to 338.07 ± 12.37 kDa. With superfine grinding, pectin extracted with different conditions presented various properties. Pectin extracted at pH 2.5 had higher esterification degree (DE, ~40%) and gelled at 3% (w/v) concentration. In contrast, pectin extracted under mild condition with low DE (21%) cannot gel at the same concentration. Conclusively, superfine grinding has potential application in pectin extraction.

Keywords: Endo-pectinases; Extraction conditions; Pectin properties; Pectin structure; Sunflower head pectin; Superfine grinding.

MeSH terms

Esterification
Helianthus / chemistry*
Hot Temperature
Molecular Weight
Pectins / chemistry*
Pectins / isolation & purification

Substances

Pectins