Skip to main content
Log in

Valuation of Citrus reticulata (kinnow) peel for the extraction of lutein using ultrasonication technique

  • Original Article
  • Published:
Biomass Conversion and Biorefinery Aims and scope Submit manuscript

Abstract

Citrus reticulata (kinnow) peel is a good source of carotenoids which can be extracted using ultrasound-assisted extraction (UAE). The present study was conducted to optimize the process parameters for the extraction of lutein using UAE method with methanolic solution of potassium hydroxide as the extractant to obtain its maximum yield. The obtained extract was used for the determination of lutein yield using high-pressure liquid chromatography (HPLC). The optimized values of process parameters in the current investigation were 6.16 mL/g solvent/solid ratio, 43.14 °C temperature, 32.88% amplitude and time duration of 33.71 min, which resulted in highest lutein yield of 29.70 μg/g. The results of this study showed that UAE system has the ability to replace the conventional techniques for extraction of lutein from citrus peels. Moreover, the extracted lutein from citrus peels can be further used as an antioxidant and colouring agent in various food applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Devkota RP, Grewal SS, Dhatt AS (1982) Maturity determination in kinnow mandarin. Punjab Hort J 22:131–135

    Google Scholar 

  2. Rafiq S, Kaul R, Sofi SA, Bashir N, Nazir F, Ahmad Nayik G (2018) Citrus peel as a source of functional ingredient: a review. J Saudi Soc Agric Sci 17:351–358

    Google Scholar 

  3. Saini A, Panesar PS, Bera MB (2019) Valorization of fruits and vegetables waste through green extraction of bioactive compounds and their nanoemulsions-based delivery system. Bioresour Bioprocess 6:1–12. https://doi.org/10.1186/s40643-019-0261-9

    Article  Google Scholar 

  4. Chhikara N, Kour R, Jaglan S, Gupta P, Gat Y, Panghal A (2018) Citrus medica: nutritional, phytochemical composition and health benefits—a review. Food Funct 9:1978–1992

    Google Scholar 

  5. Xu CJ, Fraser PD, Wang WJ, Bramley PM (2006) Differences in the carotenoid content of ordinary citrus and lycopene-accumulating mutants. J Agric Food Chem 54:5474–5481

    Google Scholar 

  6. Yen WJ, Chen BH (1995) Isolation of xanthophylls from Taiwanese orange peels and their effects on the oxidation stability of soybean oil. Food Chem 53:417–425

    Google Scholar 

  7. Fernandez-Sevilla JM, Acien Fernandez FG, Molina Grima E (2010) Biotechnological production of lutein and its applications. Appl Microbiol Biotechnol 86:27–40

    Google Scholar 

  8. Lin JH, Lee DJ, Chang JS (2015) Lutein production from biomass: marigold flowers versus microalgae. Bioresour Technol 184:421–428

    Google Scholar 

  9. Del Campo JA, Garcia-Gonzalez M, Guerrero MG (2007) Outdoor cultivation of microalgae for carotenoid production: current state and perspectives. Appl Microbiol Biotechnol 74:1163–1174

    Google Scholar 

  10. Wilkins MR, Suryawati L, Maness NO, Chrz D (2007) Ethanol production by Saccharomyces cerevisiae and Kluyveromyces marxianus in the presence of orange-peel oil. World J Microbiol Biotechnol 23:1161–1168

    Google Scholar 

  11. FAO (2014) Definitional framework of food losses and waste. Rome

  12. Panwar D, Panesar PS, Chopra HK (2019) Recent trends on the valorization strategies for the management of citrus by-products. Food Rev Int. https://doi.org/10.1080/87559129.2019.1695834

  13. Sagar NA, Pareek S, Sharma S, Yahia EM, Lobo MG (2018) Fruit and vegetable waste: bioactive compounds, their extraction, and possible utilization. Compr Rev Food Sci Food Saf 17:512–531

    Google Scholar 

  14. Altemimi A, Lightfoot DA, Kinsel M, Watson DG (2015) Employing response surface methodology for the optimization of ultrasound assisted extraction of lutein and beta-carotene from spinach. Molecules 20:6611–6625

    Google Scholar 

  15. Fu XQ, Zhang G, Deng L, Dang YY (2018) Simultaneous extraction and enrichment of polyphenol and lutein from marigold (Tagetes erecta L.) flower by enzyme-assisted ethanol/ammonium sulfate system. Food Funct 10:66–276

    Google Scholar 

  16. Gayathri S, Rajasree Radhika SR, Suman TY, Aranganathan L (2018) Ultrasound-assisted microextraction of β, ε-carotene-3, 3′-diol (lutein) from marine microalgae Chlorella Salina: effect of different extraction parameters. Biomass Convers Bior 8:791–797

    Google Scholar 

  17. Cobb BF, Kallenbach J, Hall CA, Pryor SW (2018) Optimizing the supercritical fluid extraction of lutein from corn gluten meal. Food Bioprocess Technol 11:757–764

    Google Scholar 

  18. Drosou C, Kyriakopoulou K, Bimpilas A, Tsimogiannis D, Krokida M (2015) A comparative study on different extraction techniques to recover red grape pomace polyphenols from vinification byproducts. Ind Crop Prod 75:141–149

    Google Scholar 

  19. Farahmandfar R, Esmaeilzadeh Kenari R, Asnaashari M, Shahrampour D, Bakhshandeh T (2019) Bioactive compounds, antioxidant and antimicrobial activities of Arum maculatum leaves extracts as affected by various solvents and extraction methods. Food Sci Nutr 7:465–475

    Google Scholar 

  20. Chemat F, Tomao V, Virot M (2008) Ultrasound-assisted extraction in food analysis. In: Ötleş S (ed) Handbook of food analysis instruments. CRC Press, Boca Raton, pp 85–103

    Google Scholar 

  21. Chemat F, Rombaut N, Sicaire AG, Meullemiestre A, Fabiano-Tixier AS, Abert-Vian M (2017) Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason Sonochem 34:540–560

    Google Scholar 

  22. Garrido T, Gizdavic-Nikolaidis M, Leceta I, Urdanpilleta M, Guerrero P, De la Caba K, Kilmartin PA (2019) Optimizing the extraction process of natural antioxidants from chardonnay grape marc using microwave-assisted extraction. Waste Manag 88:110–117

    Google Scholar 

  23. Wang B, Hui Y, Liu L, Zhao A, Chiou YS, Zhang F, Pan MH (2019) Optimized extraction of phenolics from jujube peel and their anti-inflammatory effects in LPS-stimulated murine macrophages. J Agric Food Chem 67:1666–1673

    Google Scholar 

  24. Derrien M, Badr A, Gosselin A, Desjardins Y, Angers P (2019) Optimization of a sustainable purification protocol for lutein and chlorophyll from spinach by-products by a saponification procedure using box Behnken design and desirability function. Food Bioprod Process 116:54–62

    Google Scholar 

  25. Talib R, Syuhadah N, Halmi MIE, Abd Ghani SS, Zaidan UH, Shukor MYA (2019) Artificial neural networks (ANNs) and response surface methodology (RSM) approach for modelling the optimization of chromium (VI) reduction by ewly isolated Acinetobacter radioresistens strain NS-MIE from agricultural soil. Biomed Res Int. https://doi.org/10.1155/2019/5785387

  26. Khan MK, Abert-Vian M, Fabiano-Tixier AS, Dangles O, Chemat F (2010) Ultrasound-assisted extraction of polyphenols (flavanone glycosides) from orange (Citrus sinensis L.) peel. Food Chem 119:851–858

    Google Scholar 

  27. Safdar MN, Kausar T, Jabbar S, Mumtaz A, Ahad K, Saddozai AA (2017) Extraction and quantification of polyphenols from kinnow (Citrus reticulate L.) peel using ultrasound and maceration techniques. J Food Drug Anal 25:488–500

    Google Scholar 

  28. Agócs A, Nagy V, Szabó Z, Márk L, Ohmacht R, Deli J (2007) Comparative study on the carotenoid composition of the peel and the pulp of different citrus species. Innov Food Sci Emerg Technol 8:390–394

    Google Scholar 

  29. Craft NE (2001) Chromatographic techniques for carotenoid separation. In: Giusti MM, Wrolstad RE (eds) Current protocols in food analytical chemistry. Wiley, Hoboken, pp F2.3.1–F2.3.15

    Google Scholar 

  30. Digesù AM, Platani C, Cattivelli L, Mangini G, Blanco A (2009) Genetic variability in yellow pigment components in cultivated and wild tetraploid wheats. J Cereal Sci 50:210–218

    Google Scholar 

  31. Varzakas T, Kiokias S (2016) HPLC analysis and determination of carotenoid pigments in commercially available plant extracts. Curr Res Nutr Food Sci 4:1–14

    Google Scholar 

  32. Xia EQ, Ai XX, Zang SY, Guan TT, Xu XR, Li HB (2011) Ultrasound-assisted extraction of phillyrin from Forsythia suspense. Ultrason Sonochem 18:549–552

    Google Scholar 

  33. Deng FG, Xu DP, Li S, Li HB (2015) Optimization of ultrasound-assisted extraction of natural antioxidants from sugar apple (Annona squamosa L.) peel using response surface methodology. Molecules 20:20448–20459

    Google Scholar 

  34. Santos HM, Lodeiro C, Capelo-Martínez JL (2009) The power of ultrasound. In: Capelo-Martínez JL (ed) Ultrasound in chemistry: analytical applications. Wiley-VCH Verlag, Weinheim, pp 1–16

    Google Scholar 

  35. Ranjan A, Patil C, Moholkar VS (2010) Mechanistic assessment of microalgal lipid extraction. Ind Eng Chem Res 49:2979–2985

    Google Scholar 

  36. Singh D, Barrow CJ, Mathur AS, Tuli DK, Puri M (2015) Optimization of zeaxanthin and β-carotene extraction from Chlorella saccharophila isolated from New Zealand marine waters. Biocatal Agric Biotechnol 4:166–173

    Google Scholar 

  37. Al-dhabi NA, Ponmurugan K, Maran P (2017) Development and validation of ultrasound-assisted solid-liquid extraction of phenolic compounds from waste spent coffee grounds. Ultrason Sonochem 34:206–213

    Google Scholar 

  38. Carrera C, Ruiz-rodríguez A, Palma M, Barroso CG (2012) Ultrasound assisted extraction of phenolic compounds from grapes. Anal Chim Acta 732:100–104

    Google Scholar 

  39. Tiwari H, Singh P, Mishra P, Srivastava P (2010) Evaluation of various techniques for extraction of natural colorants from pomegranate rind-ultrasound and enzyme assisted extraction. Indian J Fibre Text Res 35:272–276

    Google Scholar 

  40. Ghitescu RE, Volf I, Carausu C, Bühlmann AM, Gilca IA, Popa VI (2015) Optimization of ultrasound-assisted extraction of polyphenols from spruce wood bark. Ultrason Sonochem 22:535–541

    Google Scholar 

  41. Calvo MM (2005) Lutein: a valuable ingredient of fruit and vegetables. Crit Rev Food Sci Nutr 45:671–696

    Google Scholar 

  42. Ghafoor K, Choi YH, Jeon JY, Jo IH (2009) Optimization of ultrasound-assisted extraction of phenolic compounds, antioxidants, and anthocyanins from grape (Vitis vinifera) seeds. J Agric Food Chem 57:4988–4994

    Google Scholar 

  43. Tomšik A, Pavlić B, Vladić J, Ramić M, Brindza J, Vidović S (2016) Optimization of ultrasound-assisted extraction of bioactive compounds from wild garlic (Allium ursinum L.). Ultrason Sonochem 29:502–511

    Google Scholar 

  44. M’hiri N, Ioannou I, Boudhrioua NM, Ghoul M (2015) Effect of different operating conditions on the extraction of phenolic compounds in orange peel. Food Bioprod Process 6:161–170

    Google Scholar 

  45. Wang YC, Chuang YC, Hsu HW (2008) The flavonoid, carotenoid and pectin content in peels of citrus cultivated in Taiwan. Food Chem 106:277–284

    Google Scholar 

  46. Mehmood A, Ishaq M, Zhao L, Yaqoob S, Safdar B, Nadeem M, Munir M, Wang C (2019) Impact of ultrasound and conventional extraction techniques on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.). Ultrason Sonochem 51:12–19

    Google Scholar 

  47. Saini A, Panesar PS, Bera MB (2019) Comparative study on the extraction and quantification of polyphenols from citrus peels using maceration and ultrasonic technique. Curr Res Nutr Food Sci. https://doi.org/10.12944/CRNFSJ.7.3.08

  48. Song J, Yang Q, Huang W, Xiao Y, Li D, Liu C (2018) Optimization of trans lutein from pumpkin (Cucurbita moschata) peel by ultrasound-assisted extraction. Food Bioprod Process 107:104–112

    Google Scholar 

  49. Luengo E, Condón-Abanto S, Condón S, Álvarez I, Raso J (2014) Improving the extraction of carotenoids from tomato waste by application of ultrasound under pressure. Sep Purif Technol 136:130–136

    Google Scholar 

Download references

Funding

This work has not received any funding.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Parmjit Singh Panesar.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Saini, A., Panesar, P.S. & Bera, M.B. Valuation of Citrus reticulata (kinnow) peel for the extraction of lutein using ultrasonication technique. Biomass Conv. Bioref. 11, 2157–2165 (2021). https://doi.org/10.1007/s13399-020-00605-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13399-020-00605-4

Keywords

Navigation