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Enzymatic Hydrolysis Intensification of Lignocellulolytic Enzymes Through Ultrasonic Treatment

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Abstract

Ultrasound technology is often associated with harmful effects on enzyme reactions, although it is possible to improve the productivity of bioprocesses when suitable conditions are employed. Sugarcane bagasse and straw are the feedstocks widely used in Brazil for second-generation (2G) ethanol production; however, the lignocellulose biomass conversion into fermentable sugars through the enzymatic route is not yet fully optimized. Lignocellulolytic enzymes represent a significant part of the costs related to 2G ethanol production. Nonetheless, they exhibit great potential for cost reduction due to improved enzyme features: mainly increment of its activity and an increase of hydrolysis yield. This enzymatic hydrolysis of feedstock can be enhanced by green technology ultrasound application’s combined action on the enzymes and their substrates. The mixed action increases the lignocellulose saccharification; hence, it is considered a promising alternative for fermentable sugar release. The process optimization using green approaches, such as ultrasound and enzymatic treatment, can boost the sugar yield, thus emphasizing the importance of steps integration towards biomass conversion. This review attempts to provide an overview of the effects of ultrasound treatment on lignocellulolytic enzymes used in the 2G ethanol production and those of the process intensification through an unprecedented bibliometric search.

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Acknowledgements

The authors thank National Council for Scientific and Technological Development (CNPq), Research Foundation of the State of Rio Grande do Sul (FAPERGS), São Paulo Research Foundation (FAPESP), and Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil) (Finance code 001) for the financial support and Espaço da Escrita – Pró-Reitoria de Pesquisa – UNICAMP, for the language services provided.

Funding

This work was supported by FAPESP (Grant numbers 2015/20630–4, 2018/20787–9, and 2019/08542–3), FAPERGS, CAPES, and CNPq (productivity grant 307014/2020-7).

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Authors and Affiliations

  1. Bioprocess and Metabolic Engineering Laboratory, School of Food Engineering, University of Campinas – UNICAMP, Campinas, SP, Brazil

    Maria Augusta de Carvalho Silvello & Rosana Goldbeck

  2. Laboratory of Microbiology and Bioprocesses, Department of Environmental Engineering, Federal University of Fronteira Sul – UFFS, Erechim, RS, Brazil

    Aline Frumi Camargo, Thamarys Scapini & Helen Treichel

  3. Department of Civil Engineering, Pulchowk Campus, Institute of Engineering, Tribhuvan University, Pulchowk, Lalitpur, 44700, Nepal

    Shukra Raj Paudel

  4. Department of Environmental Engineering, College of Science and Technology, Korea University, Sejong, Republic of Korea

    Shukra Raj Paudel

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  1. Maria Augusta de Carvalho Silvello
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Maria Augusta de Carvalho Silvello, Aline Frumi Camargo, and Thamarys Scapini conducted the literature search. Maria Augusta de Carvalho Silvello had the idea for the article and conducted the bibliometric investigation. Shukra Raj Paudel, Helen Treichel, and Rosana Goldbeck critically revised the work.

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Correspondence to Rosana Goldbeck.

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Silvello, M.A.d., Camargo, A.F., Scapini, T. et al. Enzymatic Hydrolysis Intensification of Lignocellulolytic Enzymes Through Ultrasonic Treatment. Bioenerg. Res. 15, 875–888 (2022). https://doi.org/10.1007/s12155-021-10334-9

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