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Extraction of bioactive compounds from Alaria esculenta with pulsed electric field

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Abstract

There is a growing demand for bioactive compounds derived from green extraction methods from the food and cosmetic industries. Pulsed electric field (PEF)-assisted extraction has been applied for numerous foods but few on macroalgae. The present study examines PEF as part of the extraction process of polyphenols and carbohydrates from the brown macroalga Alaria esculenta. The effect of PEF treatment (0, 720 pulses) and ethanol concentration (0%, 15%) on the extraction of bioactive compounds from Alaria esculenta was assessed. To determine the pigment yield a second extraction was conducted with 60% ethanol or fractionated coconut oil with or without lecithin. The PEF treated aqueous extract had the highest yield for phenolic (10.4 ± 3.7 mg GAE g−1 DW) and carbohydrate content (21.4 ± 1.8 mg GE g−1 DW) as well as ferric reducing activity power and DPPH radical scavenging activity. The 60% ethanol extracts had a higher fucoxanthin content (73 ± 14 mg (100 g)−1 FD pulp) than the oil (2.1 mg (100 g)−1 FD pulp) and oil with lecithin (17 mg (100 g)−1 FD pulp) extracts. We obtained crude aqueous and low ethanolic extracts containing bioactive that show antioxidant activities, with either low temperature or PEF treatment from dried blades of Alaria esculenta. PEF treatment did not influence the pigment extraction with oil or 60% ethanol.

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Acknowledgements

This work was supported by RANNIS, the Icelandic Technology Development Fund. Authors would like that thank Dr. Birgir Jóhannesson at the Innovation Centre, Iceland, for assistance with the SEM imagining.

Funding

RANNIS, the Icelandic Technology Development Fund. Grant number 186857–0612.

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Authors and Affiliations

  1. Faculty of Food Science and Nutrition, University of Iceland, Saemundargata 2, 101, Reykjavik, Iceland

    Ragnhildur Einarsdóttir, Björn Viðar Aðalbjörnsson & Kristberg Kristbergsson

  2. TARAMAR Ehf., Miðnestorg 3, 245, Sandgerði, Iceland

    Ragnhildur Einarsdóttir, Kristín Anna Þórarinsdóttir, Guðrún Marteinsdóttir & Kristberg Kristbergsson

  3. Innovation Centre Iceland, Árleyni 8, 112, Reykjavík, Iceland

    Magnús Guðmundsson

  4. Faculty of Life and Environmental Sciences, University of Iceland, Saemundargata 2, 101, Reykjavík, Iceland

    Guðrún Marteinsdóttir

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  1. Ragnhildur Einarsdóttir
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Contributions

Ragnhildur Einarsdóttir: conceptualization, methodology, validation, formal analysis, investigation, writing–original draft, and visualisation; Kristín Anna Þórarinsdóttir: investigation, writing–review, and editing; Björn Viðar Aðalbjörnsson: supervision, writing–review, and editing; Magnús Guðmundsson: methodology and resources; Guðrún Marteinsdóttir: conceptualisation and funding acquisition; Kristberg Kristbergsson: conceptualisation, writing–review, editing, project administration, and validation.

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Einarsdóttir, R., Þórarinsdóttir, K.A., Aðalbjörnsson, B.V. et al. Extraction of bioactive compounds from Alaria esculenta with pulsed electric field. J Appl Phycol 34, 597–608 (2022). https://doi.org/10.1007/s10811-021-02624-8

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