Extraction of Polysaccharide from Dendrobium nobile Lindl. by Subcritical Water Extraction

Jing Liu; Yucheng Li; Wangqu Liu; Qi Qi; Xin Hu; Siya Li; Jiandu Lei; Long Rong

doi:10.1021/acsomega.9b02550

Extraction of Polysaccharide from Dendrobium nobile Lindl. by Subcritical Water Extraction

ACS Omega. 2019 Nov 27;4(24):20586-20594. doi: 10.1021/acsomega.9b02550. eCollection 2019 Dec 10.

Authors

Jing Liu¹, Yucheng Li¹, Wangqu Liu¹, Qi Qi¹, Xin Hu¹, Siya Li¹, Jiandu Lei¹, Long Rong²

Affiliations

¹ Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, Beijing, P. R. China.
² Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beihang University, Beijing 100191, Hebei, PR China.

Abstract

Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials. Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity. Thus, SWE of polysaccharides obtained from Dendrobium nobile Lindl. was investigated in this work. The response surface methodology (RSM) was combined with a Box-Behnken design to evaluate the influence that the three independent variables had on the response. The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure. The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%). The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled. The molecular weight, monosaccharide composition, and antioxidative activities of the polysaccharides were analyzed.