An efficient approach for simultaneously obtaining oil and epigoitrin from Orychophragmus violaceus seeds by microwave-mediated immiscible binary solvent extraction

Ru Zhao; Xinyu Yang; Aoqi Zhang; Tieyang Zhou; Yunwei Zhou; Lei Yang

doi:10.1016/j.foodchem.2021.131258

An efficient approach for simultaneously obtaining oil and epigoitrin from Orychophragmus violaceus seeds by microwave-mediated immiscible binary solvent extraction

Food Chem. 2022 Mar 15:372:131258. doi: 10.1016/j.foodchem.2021.131258. Epub 2021 Sep 30.

Authors

Ru Zhao¹, Xinyu Yang¹, Aoqi Zhang², Tieyang Zhou³, Yunwei Zhou⁴, Lei Yang⁵

Affiliations

¹ College of Horticulture, Jilin Agricultural University, Changchun 130118, China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry, University, Harbin 150040, China.
² College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry, University, Harbin 150040, China.
³ College of Horticulture, Jilin Agricultural University, Changchun 130118, China.
⁴ College of Horticulture, Jilin Agricultural University, Changchun 130118, China. Electronic address: yunweiz@jlau.edu.cn.
⁵ College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry, University, Harbin 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. Electronic address: yanglei@nefu.edu.cn.

PMID: 34627086
DOI: 10.1016/j.foodchem.2021.131258

Abstract

Microwave-mediated immiscible binary solvent extraction (MIBSE) was applied to simultaneously extract oil and epigoitrin from Orychophragmus violaceus seeds. The upper phase of n-hexane was used to obtain oil, and the lower phase of ethanol solution was used to obtain epigoitrin. Factors potentially affecting the yields of oil and epigoitrin were systematically investigated. The optimum conditions were an ethanol volume fraction of 65%, liquid-solid ratio of lower-phase of 20 mL/g, liquid-solid ratio of upper-phase of 12 mL/g, microwave irradiation power of 393 W, and microwave irradiation time of 29 min. The actual yields of oil and epigoitrin were 34.08% ± 1.38% and 11.86 ± 0.47 mg/g, respectively. GC-MS analysis illustrated that the seed oils obtained by MIBSE and Soxhlet extraction exhibited similar fatty acid compositions. The separated epigoitrin was determined by HPLC analyses, which obtained a purity of 91.25% ± 3.83%, follwed by NMR determinations.

Keywords: Epigoitrin; Microwave-mediated immiscible binary solvents extraction; Oil; Orychophragmus violaceus seeds.

MeSH terms

Microwaves*
Oils
Oxazolidinones
Plant Oils
Seeds*
Solvents

Substances

Oils
Oxazolidinones
Plant Oils
Solvents
goitrin