Abstract
The price of rice bran oil depends on the amounts of contained bioactive compounds especially oryzanol and phytosterols. The oryzanol content of rice bran oil under ohmic heating optimized at 70 V/cm, 90 °C for 3 min (27,580.75 ± 916.58 mg/kg) was higher than the control (24,908.57 ± 19.87 mg/kg). Ethanol-to-water ratio (p ≤ 0.000), medium-to-rice bran ratio (p ≤ 0.002), and electric field (p ≤ 0.028) all statistically significantly affected oryzanol content. Using water medium at low electric field (70 V/cm) produced the highest oryzanol content, while a high medium-to-rice bran ratio (1:1) resulted in the highest oryzanol content due to rapid temperature increase. Ohmic heating reduced acid value and free fatty acid content of rice bran oil and hence extended its shelf life. Ohmic heating under pressure (1 bar) was applied to elevate the boiling point and prolong the holding time, thereby obtaining higher oryzanol content (24,758.11 ± 48.96 mg/kg) than in non-pressurized rice bran oil (21,170.93 ± 42.72 mg/kg) (p ≤ 0.05). Conductivity of the medium and holding time positively correlated with oryzanol content whereas temperature had a negative correlation. Ohmic heating extraction efficiency increased with more water-soluble bioactive compounds ranking from phenolics, oryzanol, and phytosterols.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- OH:
-
Ohmic heating
- OH-Pressure:
-
Ohmic heating with pressure
- RB:
-
Rice bran
- RBO:
-
Rice bran oil
References
Aamir, M., & Jittanit, W. (2017). Ohmic heating treatment for Gac aril oil extraction: effects on extraction efficiency, physical properties and some bioactive compounds. Innovative Food Science and Emerging Technologies, 41, 224–234. https://doi.org/10.1016/j.ifset.2017.03.013.
Barba, F. J., Brianceau, S., Turk, M., Boussetta, N., & Vorobiev, E. (2015). Effect of alternative physical treatments (ultrasounds, pulsed electric fields, and high-voltage electrical discharges) on selective recovery of bio-compounds from fermented grape pomace. Food and Bioprocess Technology, 8(5), 1139–1148. https://doi.org/10.1007/s11947-015-1482-3.
Castro, I., Macedo, B., Teixeira, J. A., & Vicente, A. A. (2004). The effect of electric field on important food-processing enzymes: comparison of inactivation kinetics under conventional and ohmic heating. Journal of Food Science, 69(9), 696–701. https://doi.org/10.1111/j.1365-2621.2004.tb09918.x.
Chuang, R. L., Chen, J. C., Chu, J., & Tzen, J. T. (1996). Characterization of seed oil bodies and their surface oleosin isoforms from rice embryos. Journal of Biochemistry, 120(1), 74–81. https://doi.org/10.1093/oxfordjournals.jbchem.a021396.
Codex Alimentarius Commission. (2001). Codex Alimentarius: fats, oils and related products: Joint FAO/WHO Food Standards Programme.
Demirdoven, A., & Baysal, T. (2014). Optimization of ohmic heating applications for pectin methylesterase inactivation in orange juice. Journal of Food Science and Technology, 51(9), 1817–1826. https://doi.org/10.1007/s13197-012-0700-5.
El Darra, N., Grimi, N., Vorobiev, E., Louka, N., & Maroun, R. (2013). Extraction of polyphenols from red grape pomace assisted by pulsed ohmic heating. Food and Bioprocess Technology, 6(5), 1281–1289. https://doi.org/10.1007/s11947-012-0869-7.
Farahmandfar, R., Asnaashari, M., & Sayyad, R. (2015). Comparison antioxidant activity of Tarom Mahali rice bran extracted from different extraction methods and its effect on canola oil stabilization. Journal of Food Science and Technology, 52(10), 6385–6394. https://doi.org/10.1007/s13197-014-1702-2.
Faria, S. A. S. C., Bassinello, P. Z., & Penteado, M. V. C. (2012). Nutritional composition of rice bran submitted to different stabilization procedures. Brazilian Journal of Pharmaceutical Sciences, 48(2), 651–657. https://doi.org/10.1590/S1984-82502012000400008.
Funcia, E. S., Gut, J. A. W., & Sastry, S. (2020). Effect of electric field on pectinase inactivation during orange juice pasteurization by ohmic heating. Food and Bioprocess Technology, 13(7), 1206–1214. https://doi.org/10.1007/s11947-020-02478-x.
Goufo, P., & Trindade, H. (2014). Rice antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, gamma-oryzanol, and phytic acid. Food Science and Nutrition, 2(2), 75–104. https://doi.org/10.1002/fsn3.86.
Hoed, V. V., Depaemelaere, G., Ayala, J. V., Santiwattana, P., Verhe, R., & De Greyt, W. (2006). Influence of chemical refining on the major and minor components of rice brain oil. Journal of the American Oil Chemists' Society, 83(4), 315–321. https://doi.org/10.1007/s11746-006-1206-y.
Kim, S.M., H.J. Chung, Lim, S.T. (2014). Effect of various heat treatments on rancidity and some bioactive compounds of rice bran. Journal of Cereal Science, 60(1), 243–248.
Kumar, P., Yadav, D., Kumar, P., Panesar, P. S., Bunkar, D. S., Mishra, D., & Chopra, H. K. (2016). Comparative study on conventional, ultrasonication and microwave assisted extraction of gamma-oryzanol from rice bran. Journal of Food Science and Technology, 53(4), 2047–2053. https://doi.org/10.1007/s13197-016-2175-2.
Kumari, K., Mudgal, V., Viswasrao, G., & Srivastava, H. (2016). Studies on the effect of ohmic heating on oil recovery and quality of sesame seeds. Journal of Food Science and Technology, 53(4), 2019–2016. https://doi.org/10.1007/s13197-016-2176-1.
Kumari, B., Tiwari, B. K., Hossain, M. B., Brunton, N. P., & Rai, D. K. (2018). Recent Advances on application of ultrasound and pulsed electric field technologies in the extraction of bioactives from agro-industrial by-products. Food and Bioprocess Technology, 11(2), 223–241. https://doi.org/10.1007/s11947-017-1961-9.
Lakkakula, N. R., Lima, M., & Walker, T. (2004). Rice bran stabilization and rice bran oil extraction using ohmic heating. Bioresource Technology, 92(2), 157–161. https://doi.org/10.1016/j.biortech.2003.08.010.
Lee, J. H., Oh, S. K., Cho, K. M., Seo, W. D., & Choung, M. G. (2009). Variations in physicochemical properties of brown rice (Oryza sativa L.) during storage. Food Science and Biotechnology, 18(6), 1398–1403.
Lerma-García, M. J., Herrero-Martínez, J. M., Simó-Alfonso, E. F., Mendonça, C. R. B., & Ramis-Ramos, G. (2009). Composition, industrial processing and applications of rice bran gamma-oryzanol. Food Chemistry, 115(2), 389–404. https://doi.org/10.1016/j.foodchem.2009.01.063.
Loypimai, P., Moongngarm, A., Chottanom, P., & Moontree, T. (2015). Ohmic heating-assisted extraction of anthocyanins from black rice bran to prepare a natural food colourant. Innovative Food Science and Emerging Technologies, 27, 102–110. https://doi.org/10.1016/j.ifset.2014.12.009.
Loypimai, P., Moongngarm, A., & Chottanom, P. (2016). Phytochemicals and antioxidant capacity of natural food colorant prepared from black waxy rice bran. Food Bioscience, 15, 34–41. https://doi.org/10.1016/j.fbio.2016.04.003.
Marques, F. G., Oliveira Neto, J. R.d., Cunha, L. C.d., Paula, J. R.d., & Bara, M. T. F. (2015). Identification of terpenes and phytosterols in Dipteryx alata (baru) oil seeds obtained through pressing. Brazilian Journal of Pharmacognosy, 25(5), 522. https://doi.org/10.1016/j.bjp.2015.07.019.
Mercali, G. D., Jaeschke, D. P., Tessaro, I. C., & Marczak, L. D. (2013). Degradation kinetics of anthocyanins in acerola pulp: comparison between ohmic and conventional heat treatment. Food Chemistry, 136(2), 853–857. https://doi.org/10.1016/j.foodchem.2012.08.024.
Montesinos, L., Bundó, M., Izquierdo, E., Campo, S., Badosa, E., Rossignol, M., Montesinos, E., San Segundo, B., & Coca, M. (2016). Production of biologically active cecropin a peptide in rice seed oil bodies. PLoS One, 11(1), 1–18. https://doi.org/10.1371/journal.pone.0146919.
Murthy, P. S., & Naidu, M. M. (2010). Recovery of phenolic antioxidants and functional compounds from coffee industry by-products. Food and Bioprocess Technology, 5(3), 897–903. https://doi.org/10.1007/s11947-010-0363-z.
Nair, G. R., Divya, V. R., Prasannan, L., Habeeba, V., Prince, M. V., & Raghavan, G. S. (2014). Ohmic heating as a pre-treatment in solvent extraction of rice bran. Journal of Food Science and Technology, 51(10), 2692–2698. https://doi.org/10.1007/s13197-012-0764-2.
Pereira, R. N., Rodrigues, R. M., Genisheva, Z., Oliveira, H., de Freitas, V., Teixeira, J. A., & Vicente, A. A. (2016). Effects of ohmic heating on extraction of food-grade phytochemicals from colored potato. LWT - Food Science and Technology, 74, 493–503. https://doi.org/10.1016/j.lwt.2016.07.074.
Pestana, V. R., Zambiazi, R. C., Mendonça, C. R. B., Bruscatto, M. H., Lerma-García, M. J., & Ramis-Ramos, G. (2008). Quality changes and tocopherols and gamma-oryzanol concentrations in rice bran oil during the refining process. Journal of the American Oil Chemists' Society, 85(11), 1013–1019. https://doi.org/10.1007/s11746-008-1300-4.
Pestana, V. R., Zambiazi, R. C., Mendonça, C. R. B., Beneito-Cambra, M., & Ramis-Ramos, G. (2012). gamma-Oryzanol and tocopherol contents in residues of rice bran oil refining. Food Chemistry, 134(3), 1479–1483. https://doi.org/10.1016/j.foodchem.2012.03.059.
Piyapanrungrueang, W., Chantrapornchai, W., Haruthaithanasan, V., Sukatta, U., & Aekatasanawan, C. (2016). Comparison of anthocyanin extraction methods from high anthocyanin purple corn cob hybrid: KPSC 901, and quality of the extract powder. Journal of Food Processing and Preservation, 40(5), 1125–1133. https://doi.org/10.1111/jfpp.12693.
Pootao, S., & Kanjanapongkul, K. (2016). Effects of ohmic pretreatment on crude palm oil yield and key qualities. Journal of Food Engineering, 190, 94–100. https://doi.org/10.1016/j.jfoodeng.2016.06.021.
Pungseeklao, T., Opanasopit, P., & Khuwijitjaru, P. (2016). Development of a method for quantitative determination of gamma-oryzanol using near infrared spectroscopy. Food and Applied Bioscience Journal, 4(2), 107–115.
Ruen-Ngam, D., Thawai, C., Nokkoul, R., & Sukonthamut, S. (2014). Gamma-oryzanol extraction from upland rice bran. International Journal of Bioscience, Biochemistry and Bioinformatics, 4(4), 252–255. https://doi.org/10.7763/ijbbb.2014.v4.350.
Sakharam, K. S., Pandey, J. P., Singh, A., Kumar, A., & Shukla, A. (2016). Development of ohmic heating apparatus for extraction of rapeseed oil. International Journal for Innovative Research in Science & Technology., 2, 211–215.
Sarkis, J. R., Jaeschke, D. P., Tessaro, I. C., & Marczak, L. D. F. (2013). Effects of ohmic and conventional heating on anthocyanin degradation during the processing of blueberry pulp. LWT - Food Science and Technology, 51(1), 79–85. https://doi.org/10.1016/j.lwt.2012.10.024.
Sawadikiat, P., & Hongsprabhas, P. (2014). Phytosterols and gamma-oryzanol in rice bran oils and distillates from physical refining process. International Journal of Food Science and Technology, 49(9), 2030–2036. https://doi.org/10.1111/ijfs.12506.
Smallwood, I. M. (1996). Handbook of Organic Solvent Properties. John Wiley and Sons Inc.. https://doi.org/10.1016/C2009-0-23646-4.
Thanonkaew, A., Wongyai, S., McClements, D. J., & Decker, E. A. (2012). Effect of stabilization of rice bran by domestic heating on mechanical extraction yield, quality, and antioxidant properties of cold-pressed rice bran oil (Oryza saltiva L.). LWT - Food Science and Technology, 48(2), 231–236. https://doi.org/10.1016/j.lwt.2012.03.018.
Tzen, J. T., & Huang, A. H. (1992). Surface structure and properties of plant seed oil bodies. Journal of Cell Biology, 117(2), 327–335. https://doi.org/10.1083/jcb.117.2.327.
Verardo, V., Gómez-Caravaca, A. M., Marconi, E., Segura-Carretero, A., Garrido-Frenich, A., & Fernández-Gutiérrez, A. (2016). Determination of lipophilic and hydrophilic bioactive compounds in raw and parboiled rice bran. RSC Advances, 6(56), 50786–50796. https://doi.org/10.1039/c6ra04836f.
Acknowledgments
This research was partially supported by the Agriculture Research and Development Agency (ARDA) and the National Research Council of Thailand (NRCT). Additional thanks are due to the Program on Research Publication Graduate Students, International Affairs Division, Kasetsart University, Bangkok, Thailand, for student support.
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Sangpradab, J., Kamonpatana, P., Suwannaporn, P. et al. Ohmic Heating-Aided Mechanical Extraction of Gamma-Oryzanol and Phytosterols in Rice Bran Oil. Food Bioprocess Technol 14, 1542–1554 (2021). https://doi.org/10.1007/s11947-021-02655-6
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DOI: https://doi.org/10.1007/s11947-021-02655-6