Abstract
The concept of a biorefinery can be applied to the haskap plant (Lonicera caerulea), where the berries are harvested but the leaves are an under-utilized resource. In this study, aqueous two-phase extraction (ATPE) was used to extract chlorogenic acid (CGA) from haskap leaves. The consumption of CGA is associated with health benefits and it is sold as a natural health supplement. Two different aqueous two-phase systems (ATPSs), consisting of ethanol/sodium dihydrogen phosphate (NaH2PO4) and ethanol/potassium phosphate dibasic (K2HPO4), were compared. The factors investigated were salt/ethanol composition and extraction time. The ethanol/NaH2PO4 system had higher yield of 85.03 mg CGA/g leaves, partition coefficient of 40.41, and 92.97% extraction efficiency. The yield from conventional Soxhlet extraction was 109.74 mg CGA/g leaves; however, this required a much longer extraction time of 24 h. LC-HRMS also showed the presence of CGA, and possibly lonicerin, rutin, and diosmin isomers in the leaf extracts.
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Acknowledgements
The authors would like to thank Cynthia Swinimer of Lone Tree Farm and Dr. Evie Kemp of Haskapa for assisting in the leaf collection process.
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This work was supported by funding from the Natural Sciences and Engineering Council of Canada (NSERC).
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Linna Xie and Kar Yeen Chong are co-first authors.
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Xie, L., Chong, K.Y., Stefanova, R. et al. Recovery of chlorogenic acid from haskap leaves (Lonicera caerulea) using aqueous two-phase extraction. Biomass Conv. Bioref. 13, 3741–3750 (2023). https://doi.org/10.1007/s13399-021-01524-8
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DOI: https://doi.org/10.1007/s13399-021-01524-8